Overexpression of the poplar NF-YB7 transcription factor confers drought tolerance and improves water-use efficiency in Arabidopsis.
Overexpression of the poplar NF-YB7 transcription factor confers drought tolerance and improves water-use efficiency in Arabidopsis.
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杨树 NF-YB7 转录因子的过度表达赋予拟南芥耐旱性并提高水分利用效率
DOI：
10.1093/jxb/ert262
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Yin WL
中科院分区：
文献类型：
--
作者：
Han X;Tang S;An Y;Zheng DC;Xia XL;Yin WL
水分亏缺是全球范围内限制植物生长和生产力的一个严重环境因素。提高耐旱性和有效利用水分是克服这一困境的重要战略。本研究从速生白杨无性系NE-19中分离到一个受渗透胁迫（PEG 6000）和脱落酸诱导的干旱应答转录因子--核因子Y亚基B 7（PdNF-YB 7）。拟南芥中PdNF-YB 7（oxPdB 7）的异位过表达增强了耐旱性和整株和瞬时叶片水分利用效率（WUE，生物量与消耗的水的比率）。过表达系的发芽率和根长增加，水分散失减少，并表现出较高的光合速率、瞬时叶片WUE和叶水势，在缺水情况下表现出较强的耐旱性。此外，在拟南芥中过表达PdNF-YB 7通过增加碳同化和减少水分充足的蒸腾作用来改善全植物WUE。在正常和干旱条件下，这些耐旱、高WUE的转基因拟南芥比对照具有更早的幼苗建立和更高的生物量。相比之下，拟南芥突变体nf-yb 3对干旱胁迫更敏感，WUE较低。然而，互补分析表明，互补系（nf-yb 3/PdB 7）具有几乎相同的干旱反应和WUE的野生型Col-0。综上所述，这些结果表明PdNF-YB 7在拟南芥中积极赋予耐旱性并提高WUE;因此，它可能用于培育耐旱植物，即使在缺水的情况下也能提高产量。
Water deficit is a serious environmental factor limiting the growth and productivity of plants worldwide. Improvement of drought tolerance and efficient water use are significant strategies to overcome this dilemma. In this study, a drought-responsive transcription factor, NUCLEAR FACTOR Y subunit B 7 (PdNF-YB7), induced by osmotic stress (PEG6000) and abscisic acid, was isolated from fast-growing poplar clone NE-19 [Populus nigra × (Populus deltoides × Populus nigra)]. Ectopic overexpression of PdNF-YB7 (oxPdB7) in Arabidopsis enhanced drought tolerance and whole-plant and instantaneous leaf water-use efficiency (WUE, the ratio of biomass produced to water consumed). Overexpressing lines had an increase in germination rate and root length and decrease in water loss and displayed higher photosynthetic rate, instantaneous leaf WUE, and leaf water potential to exhibit enhanced drought tolerance under water scarcity. Additionally, overexpression of PdNF-YB7 in Arabidopsis improved whole-plant WUE by increasing carbon assimilation and reducing transpiration with water abundance. These drought-tolerant, higher WUE transgenic Arabidopsis had earlier seedling establishment and higher biomass than controls under normal and drought conditions. In contrast, Arabidopsis mutant nf-yb3 was more sensitive to drought stress with lower WUE. However, complementation analysis indicated that complementary lines (nf-yb3/PdB7) had almost the same drought response and WUE as wild-type Col-0. Taken together, these results suggest that PdNF-YB7 positively confers drought tolerance and improves WUE in Arabidopsis; thus it could potentially be used in breeding drought-tolerant plants with increased production even under water deficiency.
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