Tissue-specific, development-dependent phenolic compounds accumulation profile and gene expression pattern in tea plant [Camellia sinensis].
Tissue-specific, development-dependent phenolic compounds accumulation profile and gene expression pattern in tea plant [Camellia sinensis].
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茶树组织特异性、发育依赖的酚类化合物积累谱和基因表达模式[Camellia sinensis]
DOI：
10.1371/journal.pone.0062315
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xia T
中科院分区：
文献类型：
--
作者：
Jiang X;Liu Y;Li W;Zhao L;Meng F;Wang Y;Tan H;Yang H;Wei C;Wan X;Gao L;Xia T
茶树[Camellia sinensis（L.）在主导茶风味方面起着至关重要的作用，并对人体健康具有许多关键的药理学益处。本研究旨在研究茶树体内酚类化合物的组织特异性、发育依赖性积累模式。采用液相色谱-串联质谱技术，共定性鉴定了50种酚类化合物。其中29种酚类化合物是基于它们的裂解行为进行定量的。大部分酚类化合物在嫩叶中的含量高于茎和根中的含量，而原花青素的总量在根中出乎意料地高。通过定量实时聚合酶链反应和聚类分析，分析了参与莽草酸、苯丙烷和类黄酮途径的63个结构和调节基因的表达模式。根据基因表达模式的相似性，将其分为C1和C2两大类，C1类基因在根中的相对表达量较高，而在芽和叶中的相对表达量较低。C2-2-1和C2-2-2-1基因的表达模式可能与叶片中酚类物质的发育依赖性积累有关。酶促分析表明，儿茶素的积累同时受到儿茶素和儿茶素的影响。建议进一步研究各种基因的表达模式以及不同化合物在不同生长阶段和不同器官中含量变化的原因。
Phenolic compounds in tea plant [Camellia sinensis (L.)] play a crucial role in dominating tea flavor and possess a number of key pharmacological benefits on human health. The present research aimed to study the profile of tissue-specific, development-dependent accumulation pattern of phenolic compounds in tea plant. A total of 50 phenolic compounds were identified qualitatively using liquid chromatography in tandem mass spectrometry technology. Of which 29 phenolic compounds were quantified based on their fragmentation behaviors. Most of the phenolic compounds were higher in the younger leaves than that in the stem and root, whereas the total amount of proanthocyanidins were unexpectedly higher in the root. The expression patterns of 63 structural and regulator genes involved in the shikimic acid, phenylpropanoid, and flavonoid pathways were analyzed by quantitative real-time polymerase chain reaction and cluster analysis. Based on the similarity of their expression patterns, the genes were classified into two main groups: C1 and C2; and the genes in group C1 had high relative expression level in the root or low in the bud and leaves. The expression patterns of genes in C2-2-1 and C2-2-2-1 groups were probably responsible for the development-dependent accumulation of phenolic compounds in the leaves. Enzymatic analysis suggested that the accumulation of catechins was influenced simultaneously by catabolism and anabolism. Further research is recommended to know the expression patterns of various genes and the reason for the variation in contents of different compounds in different growth stages and also in different organs.
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DOI：
10.1016/j.chroma.2011.08.056
发表时间：
2011-10-14
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Chen H;Parks TA;Chen X;Gillitt ND;Jobin C;Sang S
通讯作者：
Sang S
影响因子：
39.2
作者：
Iso, H;Date, C;Tamakoshi, A
通讯作者：
Tamakoshi, A
影响因子：
11.6
作者：
Goodman, CD;Casati, P;Walbot, V
通讯作者：
Walbot, V
影响因子：
4.2
作者：
Crespy, V;Williamson, G
通讯作者：
Williamson, G
影响因子：
4.1
作者：
FORREST, GI;BENDALL, DS
通讯作者：
BENDALL, DS