Genetic and molecular studies on Om, a locus controlling mouse preimplantation development.
Genetic and molecular studies on Om, a locus controlling mouse preimplantation development.
复制标题
Om 的遗传和分子研究，Om 是控制小鼠植入前发育的基因座。
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
C. Babinet
中科院分区：
文献类型：
--
作者：
M. Cohen;P. Balducci;C. Kress;V. Richoux;J. Renard;C. Babinet
近年来积累的一些证据表明，核质相互作用在发育中的小鼠胚胎的形成和命运中扮演着重要的角色。早期的核移植实验表明，移植到去核受精卵后的核直接切割的能力与更高级的植入前阶段的核突然下降[1]。发生在第二个细胞周期中的核转录激活可能会阻止核在后期的分裂阶段重新编程[2]。因此，当8-细胞核被转移到去核的受精卵时，这样的重组受精卵被阻止在2-细胞阶段。然而，当将相同的8-细胞核移植到去核2-细胞胚胎的两个卵裂球中时，它们能够支持发育到囊胚期，甚至产生活的后代[2-4]。这表明细胞质环境对于传入细胞核支持发育的能力是重要的。值得注意的是，在这些实验中，核浆比例也是决定重组胚胎发育的一个重要因素[2]。在监测单倍体胚胎发育时也进行了类似的观察[5]。在另一项研究中，Latham和Solter[6]检测了雄激素--通过用雄性原核取代受精卵的雌性原核--发育到囊胚期的能力。从C57B1/6卵产生的雄激素比DBA/2产生的雄激素更有能力产生囊胚。然而，当(DBA/2×C57B1/6)F1构建雄激素时，通过用C57B1/6原核取代DBA/2雌性原核，受精卵(以下将表明胚胎的遗传构成是母本在后)，它们的发育也很差。这不仅仅是因为DBA/2细胞质中缺乏某些成分，因为将来自(DBA/2×C57B1/6)F1的C57B1/6雄性原核移植到去核的C57B1/6卵子中也观察到了发育障碍。
Several lines of evidence have accumulated in recent years indicating that nuclear cytoplasmic interactions play an important role in the formation and fate of the developing mouse embryo. Early nuclear transplantation experiments indicated that the ability of nuclei to direct cleavage after transfer into enucleated zygotes falls abruptly with nuclei from more advanced preimplantation stages [1]. Transcriptional activation of the nuclei, which occurs during the second cell cycle probably precludes the reprogramming of nuclei from later cleavage stages [2]. Thus, when an 8-cell nucleus is transferred to an enucleated zygote, such a reconstituted zygote is blocked at the 2-cell stage. However, when identical 8-cell nuclei were transferred into both blastomeres of enucleated 2-cell embryos, they were able to support development to the blastocyst stage and even gave rise to live offspring [2-4]. This indicated the importance of the cytoplasmic environment for the ability of the incoming nucleus to support development. It should be noted that in these experiments, the nuclear cytoplasmic ratio was also an important factor in determining the development of the reconstituted embryos [2]. Similar observations were also made when monitoring the development of haploid embryos [5]. In another study, Latham and Solter [6] examined the ability of androgenones, obtained by replacing the female pronucleus of a zygote by the male pronucleus, to develop to the blastocyst stage. Androgenones generated from C57B1/6 eggs were found to be much more competent to give rise to blastocysts than were DBA/2 androgenones. However, when androgenones were constructed from (DBA/2×C57B1/6)F 1 , zygotes (genetic constitution of the embryos will hereafter be indicated with the female parent coming first followed by the male parent), by replacing the DBA/2 female pronucleus with a C57B1/6 pronucleus, they also developed poorly. This was not simply due to the lack of some component in DBA/2 cytoplasm, since the impaired development was also observed when C57B1/6 male pronuclei from pairs of (DBA/2×C57B1/6) F 1 , were transferred to an enucleated C57B1/6 egg.
登录
查看更多内容
影响因子：
56.9
作者：
MCGRATH, J;SOLTER, D
通讯作者：
SOLTER, D
影响因子：
56.9
作者：
MCGRATH, J;SOLTER, D
通讯作者：
SOLTER, D
DOI：
10.1016/0165-1110(92)90029-9
发表时间：
1992-12-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
SCHULTZ, GA;HEYNER, S
通讯作者：
HEYNER, S
卵子成分对雄激素小鼠胚胎发育能力的影响。
DOI：
10.1242/dev.113.2.561
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Latham,KE;Solter,D
通讯作者：
Solter,D
小鼠 Cd7 映射到 11 号染色体。
影响因子：
3.2
作者：
Lee,DM;Watson,ML;Seldin,MF
通讯作者：
Seldin,MF