Comparative analysis of the immune responses of CcIgZ3 in mucosal tissues and the co-expression of CcIgZ3 and PCNA in the gills of common carp (Cyprinus carpio L.) in response to TNP-LPS.
Comparative analysis of the immune responses of CcIgZ3 in mucosal tissues and the co-expression of CcIgZ3 and PCNA in the gills of common carp (Cyprinus carpio L.) in response to TNP-LPS.
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DOI：
10.1186/s12917-023-03854-3
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发表时间：
2024-01-06
影响因子：
2.6
通讯作者：
Zhang, Fumiao
中科院分区：
文献类型：
--
作者：
Zhang, Jiaqi;Ren, Haoyue;Zhu, Qiannan;Kong, Xiangrui;Zhang, Feng;Wang, Chang;Wang, Yimeng;Yang, Guiwen;Zhang, Fumiao
鱼生活在富含各种微生物和病原体的水环境中。鱼粘膜相关淋巴组织(MALT)在免疫防御中起着非常重要的作用。本研究旨在研究CcIgZ3对鲤鱼(Cyprinus Carpio.)并对鳃中增殖的CcIgZ3+B淋巴细胞进行了研究。用TNP-内毒素(2，4，6-三硝基苯基半抗原与脂多糖偶联)或TNP-KLH(2，4，6-三硝基苯基半抗原与锁孔血蓝蛋白偶联)刺激鲤鱼不同组织，测定CcIgZ3在鲤鱼不同组织中的表达。与TNP-KLH相比，TNP-LPS可诱导头肾、鳃和后肠中CcIgZ3的表达，尤以鳃为甚。结果表明，鳃是CcIgZ3介导免疫应答的主要部位之一。为了检测CcIgZ3+B淋巴细胞的分布，用CcIgZ3多克隆抗体进行了免疫组织化学(IHC)实验。结果表明，在头肾、后肠和鳃中检测到CcIgZ3。为了进一步研究CcIgZ3+B淋巴细胞是否在鳃中增殖，用抗CcIgZ3多克隆抗体和抗增殖细胞核抗原单抗对增殖的CcIgZ3+B细胞进行免疫荧光染色。在3DPS和7DPS时，CcIgZ3和增殖细胞核抗原(增殖细胞核抗原)双标细胞定位于鲤鱼鳃细丝上皮细胞内，7DPS时鲤鱼鳃中出现较多增殖的CcIgZ3+B细胞。提示TNP-内毒素刺激后，鳃内CcIgZ3+B细胞可能通过局部增殖而产生。综上所述，与TNP-K1H相比，CcIgZ3在TNP-LPS攻击鲤鱼后，优先影响鲤鱼的鳃。CcIgZ3+B细胞在鳃中增殖，迅速产生CcIgZ3抗体。此外，CcIgZ3+B细胞可以被激活，在鳃的局部非常早地诱导强烈的免疫反应，并产生抗体CcIgZ3，这有助于发挥免疫保护作用。这些结果表明，可以设计一种有效的疫苗来促进粘膜抗体CcIgZ3的产生。
Fish live in an aquatic environment rich in various microorganisms and pathogens. Fish mucosal-associated lymphoid tissue (MALT) plays a very important role in immune defence. This study was conducted to characterize the immune response mediated by CcIgZ3 in common carp (Cyprinus carpio.) and investigate the proliferating CcIgZ3+ B lymphocytes in gill. We determined the expression of CcIgZ3 in many different tissues of common carp following stimulation by intraperitoneal injection of TNP-LPS (2,4,6-Trinitrophenyl hapten conjugated to lipopolysaccharide) or TNP-KLH (2,4,6-Trinitrophenyl hapten conjugated to Keyhole Limpet Hemocyanin). Compared with TNP-KLH, TNP-LPS can induce greater CcIgZ3 expression in the head kidney, gill and hindgut, especially in the gill. The results indicate that the gill is one of the main sites involved in the immune response mediated by CcIgZ3. To examine the distribution of CcIgZ3+ B lymphocytes, immunohistochemistry (IHC) experiments were performed using a polyclonal antibody against CcIgZ3. The results indicated that CcIgZ3 was detected in the head kidney, hindgut and gill. To further examine whether CcIgZ3+ B lymphocytes proliferate in the gills, proliferating CcIgZ3+ B cells were analysed by immunofluorescence staining using an anti-CcIgZ3 polyclonal antibody and an anti-PCNA monoclonal antibody. CcIgZ3 and PCNA (Proliferating Cell Nuclear Antigen) double-labelled cells in the gills were located within the epithelial cells of the gill filaments of common carp stimulated with TNP-LPS at 3 dps and 7 dps, and relatively more proliferating CcIgZ3+ B cells appeared in the gills of common carp at 7 dps. These data imply that CcIgZ3+ B cells in the gills might be produced by local proliferation following TNP-LPS stimulation. In summary, compared with those in TNP-KLH, CcIgZ3 preferentially affects the gills of common carp following challenge with TNP-LPS. CcIgZ3+ B cells proliferate in the gills to quickly produce the CcIgZ3 antibody. In addition, CcIgZ3+ B cells can be activated to induce a strong immune response very early locally in the gill and produce the antibody CcIgZ3, which helps exert an immune-protective effect. These results suggest that an effective vaccine can be designed to promote production of the mucosal antibody CcIgZ3.
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DOI：
10.1038/nrg2703
发表时间：
2010-01
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.0808182105
发表时间：
2008-10-21
影响因子：
11.1
作者：
Roa, Sergio;Avdievich, Elena;Scharff, Matthew D.
通讯作者：
Scharff, Matthew D.
影响因子：
2.8
作者：
De Miguel, Encarnacion;Alvarez-Otero, Rosa
通讯作者：
Alvarez-Otero, Rosa
影响因子：
2.9
作者：
Gambon-Deza, Francisco;Sanchez-Espinel, Christian;Magadan-Mompo, Susana
通讯作者：
Magadan-Mompo, Susana
影响因子：
4.5
作者：
Monteiro, Sandra Mariza;dos Santos, Nuno M. S.;Sousa, Mario
通讯作者：
Sousa, Mario