Phenotypic and functional heterogeneity of bovine blood monocytes.
Phenotypic and functional heterogeneity of bovine blood monocytes.
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DOI：
10.1371/journal.pone.0071502
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Schuberth HJ
中科院分区：
文献类型：
--
作者：
Hussen J;Düvel A;Sandra O;Smith D;Sheldon IM;Zieger P;Schuberth HJ
小鼠和人外周血单核细胞在大小、粒度、核形态、表型和功能上是异质的。本研究分析了牛血单核细胞是否以及如何遵循这种模式。通过流式细胞术，鉴定了经典单核细胞（cM）CD 14 + CD 16 −、中间单核细胞（intM）CD 14 + CD 16+和非经典单核细胞（ncM）CD 14 + CD 16+，其中cM是主要亚群（89%）。cM中CD 172 a表达较低，intM中MHC Ⅱ类分子表达最高，ncM中CD 163表达较低。与cM和intM相比，LPS刺激后ncM的吞噬能力显著降低，活性氧产生显著减少，CXCL 8、CXCL 1和IL-1β mRNA表达显著降低。根据LPS/ATP刺激后IL-1β的分泌，cM和intM的炎性体可被激活，而ncM则不被激活。IFNγ选择性地增加cM上CD 16的表达，并诱导cM向intM的转移。总之，牛CD 172 a阳性单核细胞定义了三个具有不同表型和功能差异的单核细胞亚群。牛cM和intM与它们的人类对应物共享同源性，而牛ncM不是炎性单核细胞。
Murine and human peripheral blood monocytes are heterogeneous in size, granularity, nuclear morphology, phenotype and function. Whether and how bovine blood monocytes follow this pattern was analyzed in this study. Flow cytometrically, classical monocytes (cM) CD14+ CD16−, intermediate monocytes (intM) CD14+ CD16+ and nonclassical monocytes (ncM) CD14+ CD16+ were identified, with cM being the predominant subset (89%). cM showed a significant lower expression of CD172a, intM expressed the highest level of MHC class II molecules, and ncM were low positive for CD163. Compared to cM and intM, ncM showed a significantly reduced phagocytosis capacity, a significantly reduced generation of reactive oxygen species, and reduced mRNA expression of CXCL8, CXCL1 and IL-1β after LPS stimulation. Based on IL-1β secretion after LPS/ATP stimulation, the inflammasome could be activated in cM and intM, but not in ncM. IFNγ increased the expression of CD16 selectively on cM and induced a shift from cM into intM in vitro. In summary, bovine CD172a-positive mononuclear cells define three monocyte subsets with distinct phenotypic and functional differences. Bovine cM and intM share homologies with their human counterparts, whereas bovine ncM are not inflammatory monocytes.
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影响因子：
2.9
作者：
Gibson, Amanda;Miah, Suhel;Werling, Dirk
通讯作者：
Werling, Dirk
影响因子：
6.4
作者：
Summerfield, A;Guzylack-Piriou, L;McCullough, KC
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影响因子：
1.8
作者：
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32.4
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Geissmann F
影响因子：
6.1
作者：
Magil, AB;Tinckam, KJ
通讯作者：
Tinckam, KJ