Efficacy of zoledronic acid in treatment of teoarthritis is dependent on the disease progression stage in rat medial meniscal tear model
Efficacy of zoledronic acid in treatment of teoarthritis is dependent on the disease progression stage in rat medial meniscal tear model
复制标题
唑来膦酸治疗骨关节炎的疗效取决于大鼠内侧半月板撕裂模型中的疾病进展阶段
DOI：
10.1038/aps.2012.28
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发表时间：
2012-05
影响因子：
8.2
通讯作者：
Nie, Shao-bo
中科院分区：
文献类型：
--
作者：
Yu, De-gang;Liu, Shen;Zhu, Zhen-an;Yu, Bo;Mao, Yuan-qing;Zhao, Xin;Wang, Xiao-qing;Ding, Hui-feng;Cao, Lei;Liu, Guang-wang;Nie, Shao-bo
目的：研究骨关节炎（OA）进展的阶段是否影响第三代双膦酸盐唑来膦酸在大鼠内侧膝关节撕裂模型中的疗效。方法：在成年雄性Sprague道利大鼠中手术诱导内侧膝关节撕裂（MMT）。在OA诱导后立即、早期（从4周开始）或晚期（从8周开始）给予唑来膦酸（ZOL，100 μg/kg，sc，每周两次）。甲苯胺蓝O染色评价关节软骨退变情况。X线微CT扫描评价软骨下骨改建。关节疼痛的测量与负重不对称有关。采用免疫荧光法检测背根神经节（DRG）降钙素基因相关肽（CGRP）的表达。结果：OA诱导后12周，软骨逐渐丢失，软骨细胞数量逐渐减少。软骨下骨改建早期（4周）表现为骨吸收增加，晚期（8周）表现为骨增生增加。立即和早期ZOL管理显着改善软骨下的微观结构参数，衰减软骨退变，减少负重不对称性和CGRP的表达，而后期ZOL管理没有显着effects.Conclusion：OA进展的阶段影响ZOL治疗关节退行性变和疼痛的疗效。为了获得最大的疗效，双膦酸盐治疗应开始在大鼠骨关节炎发病的早期阶段。
Aim:To investigate whether the stage of osteoarthritis (OA) progression influenced the efficacy of the third-generation bisphosphonate zoledronic acid in a rat medial meniscal tear model.Methods:Medial meniscal tear (MMT) was surgically induced in adult male Sprague Dawley rats. Zoledronic acid (ZOL, 100 μg/kg, sc, twice a week) was administered starting immediately, early (from 4 weeks) or late (from 8 weeks) after OA induction. The degeneration of articular cartilage was evaluated with toluidine blue O staining. Subchondral bone remodeling was evaluated with X-ray micro-CT scanning. Joint pain was measured with respect to weight-bearing asymmetry. Calcitonin gene-related peptide (CGRP) expression in dorsal root ganglia (DRGs) was examined using immunofluorescence analysis. The afferent neurons in DRGs innervating the joint were identified by retrograde labeling with fluorogold.Results:Progressive cartilage loss was observed during 12 weeks after OA induction. Subchondral bone remodeling manifested as increased bone resorption at early stage (4 weeks), but as increased bone accretion at advanced stages (8 weeks). Immediately and early ZOL administration significantly improved subchondral microstructural parameters, attenuated cartilage degeneration, reduced weight-bearing asymmetry and CGRP expression, whereas the late ZOL administration had no significant effects.Conclusion:The stage of OA progression influences the efficacy of ZOL in treating joint degeneration and pain. To obtain the maximum efficacy, bisphosphonate treatment should be initiated in rat with early stages of OA pathogenesis.
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影响因子：
--
作者：
Moisio, Kirsten;Eckstein, Felix;Chmiel, Joan S.;Guermazi, Ali;Prasad, Pottumarthi;Almagor, Orit;Song, Jing;Dunlop, Dorothy;Hudelmaier, Martin;Kothari, Ami;Sharma, Leena
通讯作者：
Sharma, Leena
DOI：
10.1016/j.berh.2009.08.004
发表时间：
2010-02-01
影响因子：
5.2
作者：
Tat, Steeve Kwan;Lajeunesse, Daniel;Martel-Pelletier, Johanne
通讯作者：
Martel-Pelletier, Johanne
DOI：
--
发表时间：
1997-10
期刊：
The Western journal of medicine
影响因子：
--
作者：
H. Holman;K. Lorig
通讯作者：
H. Holman;K. Lorig
影响因子：
7
作者：
Lindsey, CT;Narasimhan, A;Majumdar, S
通讯作者：
Majumdar, S
DOI：
10.1002/art.1790060105
发表时间：
1993-03
期刊：
Arthritis care and research : the official journal of the Arthritis Health Professions Association
影响因子：
--
作者：
Sarah E. Hampson;Russell E. Glasgow;Antonette M. Zeiss;Stephen F Birskovich;Lyn S. Foster;Alana Lines
通讯作者：
Sarah E. Hampson;Russell E. Glasgow;Antonette M. Zeiss;Stephen F Birskovich;Lyn S. Foster;Alana Lines