Cloning and characterization of a putative farnesoic acid O-methyltransferase gene from the brown planthopper, Nilaparvata lugens.

Cloning and characterization of a putative farnesoic acid O-methyltransferase gene from the brown planthopper, Nilaparvata lugens.
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DOI:
10.1673/031.010.10301
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发表时间:
2010
期刊:
Journal of insect science (Online)
影响因子:
--
通讯作者:
Liu Z
Liu Z
中科院分区:
其他
文献类型:
--
作者:
Liu S;Zhang C;Yang B;Gu J;Liu Z

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保幼激素(JH)在变态和成虫生殖过程中起着关键作用。法尼酸O-甲基转移酶(FAMeT)是JH生物合成途径中的一个重要酶,催化法尼酸(FA)甲基化为法尼酸甲酯(MF)。从水稻害虫褐飞虱Nilaparvata lugens(Stal)(半翅目:尺蛾总科)中克隆了一个全长cDNA(NlFAMeT),编码299个氨基酸。NlFAMeT与其它昆虫FAMeT显示出高氨基酸同一性(52-54%)。尽管NlFAMeT转录物在咽侧体(CA)和脑(无CA)中高度表达,但未发现NlFAMeT转录物与JH滴度之间的相关性。虽然在卵巢中仅检测到低水平的NlFAMeT转录物,但在腹部中发现高水平,并且应该在腹部中未定义的一个或多个组织中。此外,NlFAMeT转录本在雌成虫卵黄发生过程中也有正向变化。这些数据表明NlFAMeT可能不是N. lugens,但它可能在卵巢发育中发挥重要作用。它也可能是重要的,在一些未知的过程中,在一个迄今尚未确定的组织在腹部。
Juvenile hormone (JH) plays key roles in both metamorphosis and adult reproductive processes. Farnesoic acid O-methyltransferase (FAMeT) is thought to be an important enzyme in the JH biosynthetic pathway, catalyzing methylation of farnesoic acid (FA) to methyl farnesoate (MF). A full-length cDNA (NlFAMeT) encoding a 299 amino acid putative FAMeT was isolated from the brown planthopper, Nilaparvata lugens (Stal) (Hemiptera: Geometroidea), a major rice pest in many parts of Asia. NlFAMeT showed high amino acid identities (52–54%) with other insect FAMeTs. Although the NlFAMeT transcript was expressed highly in corpus allatum (CA) and brain (without CA), no correlation was found between NlFAMeT transcript and JH titers. Although only a low level of NlFAMeT transcript was detected in the ovary, a high level was found in the abdomen and should be in one or more tissues undefined in the abdomen. Also, NlFAMeT transcript had a positive change during the vitellogenesis in female adults. These data indicated that NlFAMeT might not be a key enzyme in JH synthesis in N. lugens, but that it may play an important role in the ovary development. It might also be important in some unknown process in a so-far unidentified tissue in the abdomen.
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