Capsular types of Klebsiella pneumoniae revisited by wzc sequencing.
Capsular types of Klebsiella pneumoniae revisited by wzc sequencing.
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DOI：
10.1371/journal.pone.0080670
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang JT
中科院分区：
文献类型：
--
作者：
Pan YJ;Lin TL;Chen YH;Hsu CR;Hsieh PF;Wu MC;Wang JT
胶囊是细菌中重要的毒力因子。在肺炎克雷伯菌中共鉴定出78种荚膜类型。然而，当前的输入方法存在局限性。我们在这里报告了一种新的基于扩增wzc基因可变区域的基因分型方法的发展。从76个文献记载的荚膜型参考菌株或临床菌株中扩增出wzc可变区对应的片段并进行测序。其余两种荚膜类型（参考菌株K15和K50）缺乏可扩增的wzc基因，被证明是荚膜。具有相同荚膜类型的菌株在wzc的可变区（CD1-VR2-CD2）上具有≧94%的DNA序列一致性。除K22/K37、K9/K45和K52/K79三对菌株外，不同K型菌株在该区域的DNA序列一致性<80%。菌株K22和K37具有相同的荚膜多糖合成（cps）基因，但有一个基因在单个碱基上存在差异，导致移码突变。K9和K45的wzc序列具有较高的DNA序列相似性，但在其cps簇中具有不同的基因。K52和K79具有89%的wzc序列同源性，但在DNA水平上易于区分；与K52荚膜类型相同的菌株wzc序列完全一致。采用wzc系统对29株菌血症患者进行分型。wzc DNA序列证实了28个临床分离株的荚膜类型；剩下的菌株可能代表一种新的荚膜类型。因此，wzc基因分型系统是一种简单有效的肺炎克雷伯菌荚膜分型方法。
Capsule is an important virulence factor in bacteria. A total of 78 capsular types have been identified in Klebsiella pneumoniae. However, there are limitations in current typing methods. We report here the development of a new genotyping method based on amplification of the variable regions of the wzc gene. Fragments corresponding to the variable region of wzc were amplified and sequenced from 76 documented capsular types of reference or clinical strains. The remaining two capsular types (reference strains K15 and K50) lacked amplifiable wzc genes and were proven to be acapsular. Strains with the same capsular type exhibited ≧94% DNA sequence identity across the variable region (CD1-VR2-CD2) of wzc. Strains with distinct K types exhibited <80% DNA sequence identity across this region, with the exception of three pairs of strains: K22/K37, K9/K45, and K52/K79. Strains K22 and K37 shared identical capsular polysaccharide synthesis (cps) genes except for one gene with a difference at a single base which resulted in frameshift mutation. The wzc sequences of K9 and K45 exhibited high DNA sequence similarity but possessed different genes in their cps clusters. K52 and K79 exhibited 89% wzc DNA sequence identity but were readily distinguished from each other at the DNA level; in contrast, strains with the same capsular type as K52 exhibited 100% wzc sequence identity. A total of 29 strains from patients with bacteremia were typed by the wzc system. wzc DNA sequences confirmed the documented capsular type for twenty-eight of these clinical isolates; the remaining strain likely represents a new capsular type. Thus, the wzc genotyping system is a simple and useful method for capsular typing of K. pneumoniae.
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影响因子：
3.1
作者：
MIZUTA, K;OHTA, M;KATO, N
通讯作者：
KATO, N
影响因子：
28.2
作者：
Chung, D. R.;Lee, S. S.;Park, C. K.
通讯作者：
Park, C. K.
影响因子：
9.4
作者：
CRYZ, SJ;MORTIMER, PM;GERMANIER, R
通讯作者：
GERMANIER, R
影响因子：
9.4
作者：
Brisse, S;Issenhuth-Jeanjean, S;Grimont, PAD
通讯作者：
Grimont, PAD
影响因子：
4.2
作者：
Ohmori, S;Shiraki, K;Nakano, T
通讯作者：
Nakano, T