Blended gold/MnO2@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid
Blended gold/MnO2@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid
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用于荧光和磁共振测定抗坏血酸的混合金/MnO2@BSA纳米颗粒
DOI：
10.1007/s00604-018-3205-8
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发表时间：
2019-01
影响因子：
5.7
通讯作者：
Zhang Bingbo
中科院分区：
文献类型：
--
作者：
Yu Jiani;Yang Weitao;Xing Shige;Wang Jun;Han Huanxing;Zhang Pengfei;Xiang Chenyang;Zhang Bingbo
提出了一种测定抗坏血酸(AA)的荧光与磁共振(MR)双峰检测方法。它是基于一种混合的Au/MnO2@BSA混合物的使用，该混合物是通过仿生策略制备的，在生理温度下以牛血清白蛋白(BSA)为模板。MnO2@BSA组分(复合材料的一部分)对MR不敏感，但即使在超低浓度AA的氧化还原反应中也能降解为MR活性化合物。同时，由于MnO2@BSA对周围Au@BSA(复合材料的另一部分)的荧光起猝灭作用，Au/MnO2@BSA共混物恢复了其荧光。通常在470/625纳米的激发/发射波长下测量荧光。利用MnO2与AA之间的氧化还原反应，建立了AA的双模检测方案。随着AA浓度的增加，荧光信号和MR信号均增强。AA在荧光模式下的最低检测下限为0.6%μM，在MR模式下的最低检测下限为0.4%μM。对添加AA的血清样品的分析表明，荧光模式和MR模式的回收率均可达到94%。这是使用混合纳米粒子及其固有的交叉验证规则的第一份报告。图形摘要混合Au/MnO2@BSA纳米探针的仿生合成示意图以及抗坏血酸的荧光/磁共振交叉验证双模检测。
A fluorometric and magnetic resonance (MR) dual-modal detection scheme is presented for determination of ascorbic acid (AA). It is based on the use of a blended Au/MnO2@BSA mixture that was prepared via a biomimetic strategy, using bovine serum albumin (BSA) as the template at physiological temperature. The MnO2@BSA fraction (one part of the composite) is not susceptible to MR but can be degraded to MR-active compounds upon a redox reaction with even ultralow concentrations of AA. In parallel, the blended Au/MnO2@BSA recovers its fluorescence because MnO2@BSA acts as a quencher of the fluorescence of circumjacent Au@BSA (the other part of the composite). Fluorescence typically is measured at excitation/emission wavelengths of 470/625 nm. Leveraging on this redox reaction between MnO2and AA, a dual-mode detection scheme for AA was developed. Both the fluorescence and the MR signal increase with the concentration of AA. The lowest limit for the detection of AA is 0.6 μM in the fluorometric mode and 0.4 μM in the MR mode. Analysis of AA-spiked serum samples showed that the recoveries obtained by either the fluorometric and MR mode can reach 94%. This is the first report of the use of blended nanoparticles with their inherent cross-validation regularity.Graphical abstractSchematic presentation of the biomimetic synthesis of blended Au/MnO2@BSA nanoprobes and fluorometric/MR cross-validation dual-modal detection of ascorbic acid.
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