γ-Aminobutyric acid immunoreactivity in multiple cell types of the developing rabbit retina
γ-Aminobutyric acid immunoreactivity in multiple cell types of the developing rabbit retina
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发育中的兔视网膜多种细胞类型中的γ-氨基丁酸免疫反应性
DOI：
10.1017/s0952523800002856
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发表时间：
1992
影响因子：
1.9
通讯作者：
D. Redburn
中科院分区：
文献类型：
--
作者：
E. Messersmith;D. Redburn
摘要我们先前已经证明，与成年兔相比，新生兔视网膜含有更多积累[~3H]-GABA的细胞。为了将这些神经元归类为GABA能神经元，它们还必须含有内源性GABA。我们现在报告，这些新生儿细胞群对GABA抗血清也有免疫反应。在用GABA抗血清处理的兔视网膜冰冻切片上，在出生后第1天观察到两个突触层的免疫反应过程。免疫荧光纤维的出现先于外丛状层的光感受器和双极细胞终末，出生后第5天，免疫荧光纤维的出现逐渐减少。内核层和神经节细胞层的GABA免疫阳性细胞体密度下降了50%，伴随着细胞体积的增加和其余细胞向更球形的细胞形状转变。在出生后第1天和第3天，我们还观察到具有网状细胞特征的免疫反应细胞。这种细胞类型向外网层和内网层发出分支，因此两个发育中的网状层之间的联系早在出生后1天就存在了。因此，新生兔的视网膜比成年兔有更多的内源性GABA染色的细胞。这些结果再加上我们之前的研究表明，与成人相比，新生儿中表达GABA能特性的细胞类型更多，这与GABA在发育过程中作为营养剂的一般假设是一致的。
Abstract We have previously demonstrated that the neonatal rabbit retina contains a larger complement of cells that accumulate [3H]-GABA than does the adult. In order for these neurons to be classified as GABAergic, they must also contain endogenous GABA. We now report that these same neonatal cell populations are also immunoreactive to GABA antisera. In frozen sections from rabbit retina, treated with GABA antisera, immunoreactive processes in both synaptic layers were observed at postnatal day 1. The appearance of immunofluorescent fibers precedes that of photoreceptor and bipolar cell terminals in the outer plexiform layer and is diminished by postnatal day 5. Also noted, was a 50% decrease in the density of GABA-immunoreactive cell bodies in the inner nuclear and ganglion cell layers, accompanied by an increase in cell volume and a shift toward a more spherical cell shape of the remaining cells. At postnatal day 1 and 3, we also observed immunoreactive cells having the characteristic morphology of interplexiform cells. This cell type sends branches to both the outer and inner plexiform layers, thus a morphological basis for communication between the two developing plexiform layers is present as early as postnatal day 1. Thus, retinas from neonatal rabbits have a larger complement of cells that stain for endogenous GABA than does the adult. These results coupled with our previous studies suggest that GABAergic properties are expressed by a larger number of cell types in the neonate than in the adult and are consistent with the general hypothesis that GABA functions as a trophic agent during development.
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猕猴视网膜中的丛状细胞。
影响因子：
3.4
作者：
Ryan,MK;Hendrickson,AE
通讯作者：
Hendrickson,AE
影响因子：
3.4
作者：
MOSINGER, JL;YAZULLA, S;STUDHOLME, KM
通讯作者：
STUDHOLME, KM
DOI：
10.1073/pnas.85.16.6187
发表时间：
1988-08-01
影响因子：
11.1
作者：
BRECHA, N;JOHNSON, D;WASSLE, H
通讯作者：
WASSLE, H
GABA 作为发育过程中的营养因子。
影响因子：
6.1
作者：
MadtesJr,P;Redburn,DA
通讯作者：
Redburn,DA