Antitumor effects of baicalin on ovarian cancer cells through induction of cell apoptosis and inhibition of cell migration in vitro.
Antitumor effects of baicalin on ovarian cancer cells through induction of cell apoptosis and inhibition of cell migration in vitro.
复制标题
黄芩苷通过诱导细胞凋亡和抑制细胞迁移对卵巢癌细胞的体外抗肿瘤作用
DOI：
10.3892/mmr.2017.7757
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发表时间：
2017-12
影响因子：
3.4
通讯作者：
Tian W
中科院分区：
文献类型：
--
作者：
Gao C;Zhou Y;Li H;Cong X;Jiang Z;Wang X;Cao R;Tian W
黄芩苷是从黄芩中提取的一种黄酮类化合物，具有抗炎、抗病毒、抗肿瘤等多种生物化学作用。然而，黄芩苷的抗肿瘤机制还不清楚。在本研究中，黄芩苷被证明可以抑制广泛使用的卵巢癌细胞系A2780的活力和迁移，并呈剂量依赖性。MTT法显示黄芩苷处理的卵巢癌细胞的细胞活力显著降低，而对正常卵巢细胞无影响。流式细胞仪分析表明黄芩苷通过诱导细胞凋亡抑制细胞增殖。相关的潜在机制被指出是抗凋亡蛋白B细胞淋巴瘤2凋亡调节因子的下调和caspase-3和-9的激活。此外，创伤愈合和transwell实验表明，细胞迁移能力和基质金属肽酶（MMP）-2和MMP-9的表达显着抑制细胞暴露于黄芩苷，与未经处理的细胞相比。因此，本研究表明，黄芩苷有可能被用于新的抗癌治疗制剂用于治疗卵巢癌。
Baicalin, an active flavone isolated from Scutellaria baicalensis Georgi, has been demonstrated to induce various beneficial biochemical effects such as anti-inflammatory, anti-viral, and antitumor effects. However, the antitumor mechanism of baicalin is not well understood. In the present study, baicalin was demonstrated to inhibit the viability and migration of a widely used ovarian cancer cell line, A2780, in a dose-dependent manner. MTT assays revealed that cell viability significantly decreased in ovarian cancer cells treated with baicalin compared with untreated cells, without effect on normal ovarian cells. Flow cytometric analysis indicated that baicalin suppressed cell proliferation by inducing apoptosis. The underlying mechanisms involved were indicated to be downregulation of the anti-apoptotic protein B-cell lymphoma 2 apoptosis regulator and activation of caspase-3 and −9. In addition, wound healing and transwell assays revealed that cell migratory potential and expression of matrix metallopeptidase (MMP)-2 and MMP-9 were significantly inhibited when cells were exposed to baicalin, compared with untreated cells. The present study therefore suggested that baicalin has the potential to be used in novel anti-cancer therapeutic formulations for treatment of ovarian cancer.
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影响因子：
7.2
作者：
Wang, Congpin;Wang, Bin
通讯作者：
Wang, Bin
影响因子：
3.2
作者：
Ye, Chun;Li, Sali;Hou, Yongqing
通讯作者：
Hou, Yongqing
影响因子：
2.9
作者：
Fujiwara Y;Takaishi K;Nakao J;Ikeda T;Katabuchi H;Takeya M;Komohara Y
通讯作者：
Komohara Y
影响因子：
3.4
作者：
Kim, Min Kyoung;Choi, Hyeong Sim;Ko, Seong-Gyu
通讯作者：
Ko, Seong-Gyu
影响因子：
1.8
作者：
Ahmed, Khadija Muhamed
通讯作者：
Ahmed, Khadija Muhamed