Immunogenicity of two FMDV nonameric peptides encapsulated in liposomes in mice and the protective efficacy in guinea pigs.
Immunogenicity of two FMDV nonameric peptides encapsulated in liposomes in mice and the protective efficacy in guinea pigs.
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两种口蹄疫病毒非对称肽脂质体对小鼠的免疫原性及对豚鼠的保护作用
DOI：
10.1371/journal.pone.0068658
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li XS
中科院分区：
文献类型：
--
作者：
Gao FS;Feng L;Zhang Q;Yan RQ;Li YG;Li XS
口蹄疫病毒（FMDV） VP1衣壳蛋白非聚肽I （VP126-34， RRQHTDVSF）、II （VP1157-165， RTLPTSFNY）和III （VP145-53， KEQVNVLDL）被预测为T细胞表位。为了研究这些肽是否具有免疫活性，我们用结合免疫刺激复合物（ISCOMs）的肽I、II或III免疫BALB/c小鼠。采用细胞毒性T淋巴细胞试验，通过测定多肽特异性T细胞增殖和全血CD8+ T淋巴细胞数量以及多肽诱导的外周血单个核细胞干扰素(IFN)-γ的产生来评价多肽诱导的细胞毒性活性。为了进一步确定多肽的保护作用，在豚鼠中进行了FMDV攻击试验。与对照组相比，肽I和肽II刺激T细胞增殖、CD8+ T淋巴细胞、IFN-γ分泌和细胞毒活性显著增加。FMDV攻毒实验表明，肽I和肽II可以保护60%以上的动物免受病毒攻击。结果表明，包裹在脂质体中的肽I和肽II可能是FMDV的CTL表位，并能在一定程度上保护动物免受病毒的攻击。
It has been predicted that nonameric peptides I (VP126–34, RRQHTDVSF), II (VP1157–165, RTLPTSFNY) and III (VP145–53, KEQVNVLDL) from the VP1 capsid protein of the foot-and-mouth disease virus (FMDV) are T cell epitopes. To investigate whether these peptides have immunological activity, BALB/c mice were immunized with peptide I, II or III conjugated with immunostimulating complexes (ISCOMs). A cytotoxic T lymphocyte assay was used to evaluate the cytotoxic activity induced by peptides along with by measuring peptide-specific T-cell proliferation and CD8+ T lymphocyte numbers in whole blood and interferon (IFN)-γ production in peripheral blood mononuclear cells induced by peptides. To further identify the protective efficacy of peptides, an FMDV challenge assay was done in guinea pigs. Peptides I and II stimulated significant increases in T-cell proliferation, CD8+ T lymphocytes, and IFN-γ secretion and cytotoxic activity compared to controls. The FMDV challenge assay indicated peptides I and II can protect over 60% of animals from virus attack. The results demonstrate that peptides I and II encapsulated in liposomes should be CTL epitopes of FMDV and can protect animals from virus attack to some extent.
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影响因子：
6.2
作者：
Castellanos, MR;Weinstein, G;Hayes, RL
通讯作者：
Hayes, RL
影响因子：
3.3
作者：
Ercolini, Anne M.;Croxford, J. Ludovic;Miller, Stephen D.
通讯作者：
Miller, Stephen D.
影响因子：
1.8
作者：
Gao, Feng-Shan;Fang, Qin-Mei;Xia, Chun
通讯作者：
Xia, Chun
影响因子：
64.8
作者：
BITTLE, JL;HOUGHTEN, RA;BROWN, F
通讯作者：
BROWN, F
影响因子：
56.9
作者：
Altman, JD;Moss, PAH;Davis, MM
通讯作者：
Davis, MM