Genome-wide identification and expression analysis of AUX/LAX family genes in Chinese hickory (Carya cathayensis Sarg.) Under various abiotic stresses and grafting.
Genome-wide identification and expression analysis of AUX/LAX family genes in Chinese hickory (Carya cathayensis Sarg.) Under various abiotic stresses and grafting.
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DOI：
10.3389/fpls.2022.1060965
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发表时间：
2022
影响因子：
5.6
通讯作者：
He, Yi
中科院分区：
文献类型：
--
作者：
Yang, Ying;Wang, Jiayan;Xu, Yan;Abbas, Farhat;Xu, Dongbin;Tao, Shenchen;Xie, Xiaoting;Song, Feng;Huang, Qiaoyu;Sharma, Anket;Zheng, Luqing;Yan, Daoliang;Wang, Xiaofei;Zheng, Bingsong;Yuan, Huwei;Wu, Rongling;He, Yi
生长素对于调节植物的生长发育以及植物对非生物胁迫的反应是必不可少的。AUX/LAX蛋白是一种生长素内流转运蛋白，属于氨基酸渗透酶质子驱动转运蛋白家族，参与吲哚-3-乙酸(IAA)的转运。然而，关于中国山核桃AUX/LAX基因如何响应非生物胁迫的研究较少。首次利用基因克隆和实时荧光定量聚合酶链式反应技术对中国山核桃AUX/LAX基因家族进行了鉴定、结构特征和基因表达分析。在中国山核桃中鉴定出8个CcAUX/LAXs，它们都具有AUX/LAXs保守的结构特征。CcAUX/LAXs与毛果杨中CcAUX/LAXs的同源蛋白关系最为密切，这与它们在木本植物上的共同分类特征是一致的。CcAUX/LAXs在不同的组织中表现出不同的表达谱，表明它们在生长发育中的作用不同。在CcAUX/LAX基因的启动子上检测到许多光、激素和非生物胁迫反应顺式作用调控元件。CcAUX/LAX基因对干旱和盐胁迫的响应程度不同。此外，CcAUX/LAX基因在中国山核桃嫁接过程中表现出复杂的表达变化。这些发现不仅为进一步验证CcAUX/LAXs的功能提供了有价值的资源，而且有助于更好地理解它们在中国山核桃嫁接和非生物胁迫处理中的潜在调控功能。
Auxin is essential for regulating plant growth and development as well as the response of plants to abiotic stresses. AUX/LAX proteins are auxin influx transporters belonging to the amino acid permease family of proton-driven transporters, and are involved in the transport of indole-3-acetic acid (IAA). However, how AUX/LAX genes respond to abiotic stresses in Chinese hickory is less studied. For the first time identification, structural characteristics as well as gene expression analysis of the AUX/LAX gene family in Chinese hickory were conducted by using techniques of gene cloning and real-time fluorescent quantitative PCR. Eight CcAUX/LAXs were identified in Chinese hickory, all of which had the conserved structural characteristics of AUX/LAXs. CcAUX/LAXs were most closely related to their homologous proteins in Populus trichocarpa , which was in consistence with their common taxonomic character of woody trees. CcAUX/LAXs exhibited different expression profiles in different tissues, indicating their varying roles during growth and development. A number of light-, hormone-, and abiotic stress responsive cis-acting regulatory elements were detected on the promoters of CcAUX/LAX genes. CcAUX/LAX genes responded differently to drought and salt stress treatments to varying degrees. Furthermore, CcAUX/LAX genes exhibited complex expression changes during Chinese hickory grafting. These findings not only provide a valuable resource for further functional validation of CcAUX/LAXs, but also contribute to a better understanding of their potential regulatory functions during grafting and abiotic stress treatments in Chinese hickory.
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影响因子：
20.5
作者：
Korver RA;Koevoets IT;Testerink C
通讯作者：
Testerink C
影响因子：
5.6
作者：
Chai C;Subudhi PK
通讯作者：
Subudhi PK
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作者：
Assuncao, M.;Santos, C.;Fevereiro, P.
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作者：
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影响因子：
56.9
作者：
Bennett, MJ;Marchant, A;Feldmann, KA
通讯作者：
Feldmann, KA