Effects of TGF-beta3 and preculture period of osteogenic cells on the chondrogenic differentiation of rabbit marrow mesenchymal stem cells encapsulated in a bilayered hydrogel composite.
Effects of TGF-beta3 and preculture period of osteogenic cells on the chondrogenic differentiation of rabbit marrow mesenchymal stem cells encapsulated in a bilayered hydrogel composite.
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DOI：
10.1016/j.actbio.2010.02.046
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发表时间：
2010-08
影响因子：
9.7
通讯作者：
Mikos, A. G.
中科院分区：
文献类型：
--
作者：
Guo, X.;Liao, J.;Park, H.;Saraf, A.;Raphael, R. M.;Tabata, Y.;Kasper, F. K.;Mikos, A. G.
在这项工作中，可注射的，可生物降解的水凝胶复合材料的交联低聚（聚（乙二醇）富马酸酯）（OPF）和明胶微粒（MP）被用来制造一个双层的骨软骨结构。将转化生长因子-β3（TGF-β3）负载的MPs包埋于成软骨层，并与成骨前培养不同时期（0、3、6、12 d）的成骨细胞共培养，观察TGF-β3的转染及共培养对成骨层细胞增殖和分化的影响。结果显示，在软骨形成层，TGF-β3能显著促进MSCs向软骨细胞分化。此外，成骨层中不同成骨预培养期的细胞，沿着TGF-β3，在不同程度上增强MSC软骨形成标志物的基因表达。在成骨层中，细胞在共培养期间保持其碱性磷酸酶活性;然而，TGF-β3的存在延迟了矿化。总之，这项研究表明，制造双层水凝胶复合材料，模仿骨软骨组织的结构和功能，沿着这些复合材料作为细胞和生长因子载体的应用，同时说明，不同程度的成骨分化的包囊细胞可以显着影响共培养的祖细胞在存在和不存在的软骨生长因子的软骨分化。
In this work, injectable, biodegradable hydrogel composites of crosslinked oligo(poly(ethylene glycol) fumarate) (OPF) and gelatin microparticles (MPs) were utilized to fabricate a bilayered osteochondral construct. Rabbit marrow mesenchymal stem cells (MSCs) were encapsulated with transforming growth factor-β3 (TGF-β3)-loaded MPs in the chondrogenic layer and cocultured with cells of different periods of osteogenic preculture (0, 3, 6 and 12 days) in the osteogenic layer to investigate the effects of TGF-β3 delivery and coculture on the proliferation and differentiation of cells in both layers. The results showed that, in the chondrogenic layer, TGF-β3 significantly stimulated chondrogenic differentiation of MSCs. Additionally, cells of various osteogenic preculture periods in the osteogenic layer, along with TGF-β3, enhanced gene expression for MSC chondrogenic markers to different extents. In the osteogenic layer, cells maintained their alkaline phosphatase activity during the coculture; however, mineralization was delayed by the presence of TGF-β3. Overall, this study demonstrated the fabrication of bilayered hydrogel composites that mimic the structure and function of osteochondral tissue, along with the application of these composites as cell and growth factor carriers, while illustrating that encapsulated cells of different degrees of osteogenic differentiation can significantly influence the chondrogenic differentiation of cocultured progenitor cells in both the presence and absence of chondrogenic growth factors.
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影响因子：
10.8
作者：
Holland, TA;Tabata, Y;Mikos, AG
通讯作者：
Mikos, AG
影响因子：
6.2
作者：
Park, Hansoo;Guo, Xuan;Temenoff, Johnna S.;Tabata, Yasuhiko;Caplan, Arnold I.;Kasper, F. Kurtis;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
影响因子：
14
作者：
Chung, Cindy;Beecham, Michael;Mauck, Robert L.;Burdick, Jason A.
通讯作者：
Burdick, Jason A.
影响因子：
2.9
作者：
Moioli, Eduardo K.;Hong, Liu;Mao, Jeremy J.
通讯作者：
Mao, Jeremy J.
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ