Colour bio-factories: Towards scale-up production of anthocyanins in plant cell cultures.
Colour bio-factories: Towards scale-up production of anthocyanins in plant cell cultures.
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DOI：
10.1016/j.ymben.2018.06.004
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发表时间：
2018-07
影响因子：
8.4
通讯作者：
Matros A
中科院分区：
文献类型：
--
作者：
Appelhagen I;Wulff-Vester AK;Wendell M;Hvoslef-Eide AK;Russell J;Oertel A;Martens S;Mock HP;Martin C;Matros A
花色素苷是一种广泛分布的糖基化水溶性植物色素，使许多水果和花卉呈现红色、紫色或蓝色。它们在饮食环境中的有益效果鼓励了越来越多地使用花青素作为食品和化妆品工业中的天然着色剂。然而，商业上花青素苷的有限可用性和多样性已经开始寻找这些天然着色剂的替代来源。在植物中，通过调控基因以及编码生物合成酶的基因的工程化，可以实现次级代谢产物如花青素的高水平生产。我们已经使用组成型产生高水平矢车菊素3-O-芸香糖苷、飞燕草素3-O-芸香糖苷或新的花青素苷、酰化矢车菊素3-O-（香豆酰基）芸香糖苷的烟草品系来产生细胞悬浮培养物。该细胞系在其生产速率方面是稳定的，并且优于常规植物细胞培养物上级。已经证明了在小规模发酵罐中花青素苷生产的规模扩大。细胞培养物也被证明是一个合适的系统，用于生产13 C-标记的花青素。我们的花青素生产方法可转移到其他植物物种，如拟南芥，证明了这种方法用于制备各种高度装饰的花青素的潜力。烟草细胞培养物代表了传统花青素生产平台的可定制和可持续的替代品，并且在花青素的工业和医疗应用中具有相当大的潜力。我们已经开发了烟草细胞培养物，作为传统花青素生产平台的可定制和可持续的替代品，通过工程表达调控基因以及表达编码侧链装饰酶的基因。已经开发了用于可定制的花青素苷生产的烟草细胞培养物。不同的装饰和13 C标记的花青素已经产生。花青素苷产量已被证明是稳定的，高达30毫克每克干重。已经证明了在小规模发酵罐中按比例放大花青素苷生产和纯化。该方法可转移到其他物种用于生产高度装饰的花青素。
Anthocyanins are widely distributed, glycosylated, water-soluble plant pigments, which give many fruits and flowers their red, purple or blue colouration. Their beneficial effects in a dietary context have encouraged increasing use of anthocyanins as natural colourants in the food and cosmetic industries. However, the limited availability and diversity of anthocyanins commercially have initiated searches for alternative sources of these natural colourants. In plants, high-level production of secondary metabolites, such as anthocyanins, can be achieved by engineering of regulatory genes as well as genes encoding biosynthetic enzymes. We have used tobacco lines which constitutively produce high levels of cyanidin 3-O-rutinoside, delphinidin 3-O-rutinoside or a novel anthocyanin, acylated cyanidin 3-O-(coumaroyl) rutinoside to generate cell suspension cultures. The cell lines are stable in their production rates and superior to conventional plant cell cultures. Scale-up of anthocyanin production in small scale fermenters has been demonstrated. The cell cultures have also proven to be a suitable system for production of 13C-labelled anthocyanins. Our method for anthocyanin production is transferable to other plant species, such as Arabidopsis thaliana, demonstrating the potential of this approach for making a wide range of highly-decorated anthocyanins. The tobacco cell cultures represent a customisable and sustainable alternative to conventional anthocyanin production platforms and have considerable potential for use in industrial and medical applications of anthocyanins. We have developed tobacco cell cultures as customisable and sustainable alternatives to conventional anthocyanin production platforms by engineering expression of regulatory genes together with expression of genes encoding side chain decorating enzymes. Tobacco cell cultures for customisable anthocyanin production have been developed. Differentially decorated and 13C-labelled anthocyanins have been produced. Anthocyanin yield has been shown to be stable, up to 30 mg per g dry weight. Scale-up anthocyanin production in small scale fermenters and purification have been demonstrated. The approach is transferrable to other species for production of highly decorated anthocyanins.
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DOI：
10.1016/j.cub.2017.02.027
发表时间：
2017-04-03
期刊：
Current biology : CB
影响因子：
--
作者：
Kallam K;Appelhagen I;Luo J;Albert N;Zhang H;Deroles S;Hill L;Findlay K;Andersen ØM;Davies K;Martin C
通讯作者：
Martin C
影响因子：
18
作者：
Bally, Julia;Nakasugi, Kenlee;Waterhouse, Peter M.
通讯作者：
Waterhouse, Peter M.
DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
影响因子：
4.2
作者：
Espley, Richard V.;Butts, Christine A.;Hellens, Roger P.
通讯作者：
Hellens, Roger P.
影响因子：
3
作者：
Hvoslef-Eide, Anne Kathrine;Olsen, Odd Arild S.;Heyerdahl, Petter H.
通讯作者：
Heyerdahl, Petter H.