Genetic diversity among Toxoplasma gondii isolates from different hosts and geographical locations revealed by analysis of ROP13 gene sequences
Genetic diversity among Toxoplasma gondii isolates from different hosts and geographical locations revealed by analysis of ROP13 gene sequences
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通过 ROP13 基因序列分析揭示不同宿主和地理位置的弓形虫分离株的遗传多样性
DOI：
10.5897/ajb12.056
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发表时间：
2012-03
影响因子：
--
通讯作者：
RQ Lin
中科院分区：
文献类型：
--
作者：
PY Wang;P Lu;MJ Xu;J Li;DH Zhou;JF Yang;ZX Ni;ZG Yuan;RQ Lin
弓形虫可以感染几乎所有的温血动物和人类，在世界范围内造成严重的公共卫生问题和经济损失。棒状体蛋白13（ROP 13）在T.刚地。本研究对14株T.对来自不同地理位置和宿主的弓形虫分离株进行了检查。ROP13基因从单个分离株中扩增并测序。结果表明，ROP13基因全长为1203 bp。ROP 13序列共有44个变异位点，14个供试T.弓形虫分离株，颠换率高于转换率。种内变异主要发生在第二密码子位置。对14个供试的T. ROP13序列不适合作为区分弓形虫的遗传标记。来自不同宿主和地理区域的不同基因型弓形虫分离株。ROP 13基因序列变异小，提示ROP 13基因可能是一种良好的弓形虫病疫苗候选基因。关键词：弓形虫，弓形虫病，重组蛋白13，序列变异，系统发育分析。
Toxoplasma gondii can infect almost all the warm-blooded animals and human beings, causing serious public health problems and economic losses worldwide. Rhoptry protein 13 (ROP13) plays some roles in the invasion process of T. gondii . In this study, sequence variation in ROP13 gene among 14 T. gondii isolates from different geographical locations and hosts was examined. The ROP13 gene was amplified from individual isolates and sequenced. Results show that the length of the ROP13 sequences was 1203 bp. In total, there were 44 variable nucleotide positions in the ROP13 sequences, and sequence variations were 0.1 to 2.0% among the 14 examined T. gondii isolates, representing higher rate in transversion than in transition. Intra-specific nucleotide variations were mainly at the second codon positions. Phylogenetic analysis of the 14 examined T. gondii isolates indicate that the ROP13 sequence was not a suitable genetic marker to differentiate T. gondii isolates of different genotypes from different hosts and geographical regions. Low variation in ROP13 gene sequence may suggest that ROP13 gene could represent a good vaccine candidate against toxoplasmosis. Key words: Toxoplasma gondii , toxoplasmosis, rhpotry protein 13 (ROP13), sequence variation, phylogenetic analysis.
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影响因子：
10.7
作者：
Strimmer, K;vonHaeseler, A
通讯作者：
vonHaeseler, A
影响因子：
1.9
作者：
Zhou, Peng;Zhang, Han;Zhu, Xing-Quan
通讯作者：
Zhu, Xing-Quan
影响因子：
4.8
作者：
Bradley, PJ;Ward, C;Boothroyd, JC
通讯作者：
Boothroyd, JC
影响因子：
4
作者：
Grimwood, J;Smith, JE
通讯作者：
Smith, JE
影响因子：
3.2
作者：
Zhou P;Chen Z;Li HL;Zheng H;He S;Lin RQ;Zhu XQ
通讯作者：
Zhu XQ