3D Bioprinting of Cell-Laden Hydrogels for Improved Biological Functionality.
3D Bioprinting of Cell-Laden Hydrogels for Improved Biological Functionality.
复制标题
DOI：
10.1002/adma.202103691
复制
发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Heilshorn SC
中科院分区：
文献类型：
--
作者：
Hull SM;Brunel LG;Heilshorn SC
Please try later.
The encapsulation of cells within gel-phase materials to form bioinks offers distinct advantages for next-generation three-dimensional (3D) bioprinting. 3D bioprinting has emerged as a promising tool for patterning cells, but the technology remains limited in its ability to produce bio-functional, tissue-like constructs due to a dearth of materials suitable for bioinks. While early demonstrations commonly used viscous polymers optimized for printability, these materials often lacked cell compatibility and biological functionality. In response, advanced materials that exist in the gel-phase during the entire printing process are being developed, since hydrogels are uniquely positioned to both protect cells during extrusion as well as provide biological signals to embedded cells as the construct matures during culture. Here we present an overview of the design considerations for gel-phase materials as bioinks, with a focus on their mechanical, biochemical, and dynamic gel properties. We also evaluate current challenges and opportunities that arise due to the fact that bioprinted constructs are active, living hydrogels composed of both acellular and cellular components. Engineering hydrogels with consideration of cells as an intrinsic component of the printed bioink will enable control over the evolution of the living construct after printing to achieve greater bio-functionality. This review describes recent progress and future opportunities to design dual-stage bioinks, which are gels both prior to and after printing. These active, living materials are composed of both acellular and cellular components. Engineering hydrogels with consideration of cells as an intrinsic component of the printed bioink will enable greater bio-functionality.
登录
查看更多内容
影响因子：
16.6
作者：
Chaudhuri O;Gu L;Darnell M;Klumpers D;Bencherif SA;Weaver JC;Huebsch N;Mooney DJ
通讯作者：
Mooney DJ
影响因子：
41.2
作者：
Chaudhuri O;Gu L;Klumpers D;Darnell M;Bencherif SA;Weaver JC;Huebsch N;Lee HP;Lippens E;Duda GN;Mooney DJ
通讯作者：
Mooney DJ
影响因子：
10
作者：
Ali, Mohamed;Kumar, Anil P. R.;Lee, Sang Jin
通讯作者：
Lee, Sang Jin
影响因子：
13.6
作者：
Bhattacharjee T;Zehnder SM;Rowe KG;Jain S;Nixon RM;Sawyer WG;Angelini TE
通讯作者：
Angelini TE
影响因子：
21.3
作者：
Balaban, NQ;Schwarz, US;Geiger, B
通讯作者：
Geiger, B