Molecular Typing of Pathogenic Leptospira Serogroup Icterohaemorrhagiae Strains Circulating in China during the Past 50 Years.
Molecular Typing of Pathogenic Leptospira Serogroup Icterohaemorrhagiae Strains Circulating in China during the Past 50 Years.
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近50年来我国流行的致病性钩端螺旋体黄疸出血群血清群的分子分型
DOI：
10.1371/journal.pntd.0003762
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发表时间：
2015-05
影响因子：
3.8
通讯作者：
Jiang X
中科院分区：
文献类型：
--
作者：
Zhang C;Yang H;Li X;Cao Z;Zhou H;Zeng L;Xu J;Xu Y;Chang YF;Guo X;Zhu Y;Jiang X
研究背景钩端螺旋体病是世界范围内被忽视的重要热带传染病之一。黄疸出血群在中国一直是且仍然是该病原体的主要血清群。然而，关于该血清群的血清型或基因型的细节知之甚少。方法/主要发现在这项研究中，收集了来自中国五个不同地理区域的120株流行菌株，并通过16 S rRNA测序和MLST分析鉴定了8株国际参考菌株。115株、11株和2株被鉴定为L.问号湖borgpetersenii和L. kirschneri，分别。共鉴定出17株不同的ST，其中ST 1型69株，ST 17型18株，ST 128型18株，ST 143型9株，ST 209型2株。其余12个菌株属于12个不同的ST。eBURST分析表明，在分离的克隆复合物（CC）中，CC 1占73.3%（88/120），代表三种ST：ST 1，ST 128和ST 98。ST 1是该CC最可能的祖先株，其次是单细胞CC 17（17/120）和CC 143（11/120）。在MLST数据库中添加116个黄疸出血群菌株的进一步分析以及先前使用全球eBURST分析和MST树状图描述的研究显示，在这244个菌株中，ST聚类模式相对相似，有5个主要CC和8个单例。CC 17是世界范围内流行的致病性钩端螺旋体的最流行克隆。据我们所知，这是第一次，ST 1和ST 17菌株分布在4个不同的血清型，表明血清型和ST之间的关系非常复杂。结论/意义我们的研究表明，黄疸出血群菌株的遗传多样性水平高。与其他地方流行的ST 17或ST 37不同，CC 1中的ST 1在过去50年左右被证明是中国分离的致病性钩端螺旋体中最流行的ST。此外，ST和血清型之间的复杂关系表明迫切需要开发一种改进的钩体血清分型方案。
Background Leptospirosis is one of the most important neglected tropical infectious diseases worldwide. Icterohaemorrhagiae has been throughout recent history, and still is, the predominant serogroup of this pathogen in China. However, very little in detail is known about the serovars or genotypes of this serogroup. Methodology/Principal Findings In this study, 120 epidemic strains from five geographically diverse regions in China collected over a 50 year period (1958~2008), and 8 international reference strains characterized by 16S rRNA sequencing and MLST analysis. 115, 11 and 2 strains were identified as L. interrogans, L. borgpetersenii, and L. kirschneri, respectively. 17 different STs were identified including 69 ST1 strains, 18 ST17, 18 ST128, 9 ST143 and 2 ST209. The remaining 12 strains belonged to 12 different STs. eBURST analysis demonstrated that, among the clonal complexes isolated (CCs), CC1 accounted for 73.3% (88/120) strains representing three STs: ST1, ST128 and ST98. ST1 was the most likely ancestral strain of this CC, followed by singleton CC17 (17/120) and CC143 (11/120). Further analysis of adding 116 serogroup Icterohaemorrhagiae strains in the MLST database and studies previously described using global eBURST analysis and MST dendrogram revealed relatively similar ST clustering patterns with five main CCs and 8 singletons among these 244 strains. CC17 was found to be the most prevalent clone of pathogenic Leptospira circulating worldwide. This is the first time, to our knowledge, that ST1 and ST17 strains were distributed among 4 distinct serovars, indicating a highly complicated relationship between serovars and STs. Conclusions/Significance Our studies demonstrated a high level of genetic diversity in the serogroup Icterohaemorrhagiae strains. Distinct from ST17 or ST37 circulating elsewhere, ST1 included in CC1, has over the past 50 years or so, proven to be the most prevalent ST of pathogenic leptospires isolated in China. Moreover, the complicated relationship between STs and serovars indicates an urgent need to develop an improved scheme for Leptospira serotyping.
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DOI：
10.1111/1469-0691.12714
发表时间：
2014-12
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
van Duin D;Cober ED;Richter SS;Perez F;Cline M;Kaye KS;Kalayjian RC;Salata RA;Evans SR;Fowler VG Jr;Bonomo RA
通讯作者：
Bonomo RA
影响因子：
2.4
作者：
Romero, E. C.;Blanco, R. M.;Galloway, R. L.
通讯作者：
Galloway, R. L.
DOI：
10.1099/ijs.0.047324-0
发表时间：
2013-05-01
影响因子：
2.8
作者：
Smythe, L.;Adler, B.;Levett, P. N.
通讯作者：
Levett, P. N.
影响因子：
--
作者：
Voronina OL;Kunda MS;Aksenova EI;Ryzhova NN;Semenov AN;Petrov EM;Didenko LV;Lunin VG;Ananyina YV;Gintsburg AL
通讯作者：
Gintsburg AL
影响因子：
3.8
作者：
Thaipadungpanit J;Wuthiekanun V;Chierakul W;Smythe LD;Petkanchanapong W;Limpaiboon R;Apiwatanaporn A;Slack AT;Suputtamongkol Y;White NJ;Feil EJ;Day NP;Peacock SJ
通讯作者：
Peacock SJ