Multiple types of Ca2+ channels in visceral smooth muscle cells.

Multiple types of Ca2+ channels in visceral smooth muscle cells.
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内脏平滑肌细胞中多种类型的 Ca2 通道。

DOI:
10.1007/bf00370463
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发表时间:
1991
期刊:
Pflugers Archiv : European journal of physiology
影响因子:
--
通讯作者:
WalshJr,JV
WalshJr,JV
中科院分区:
--
文献类型:
--
作者:
Vivaudou,MB;Singer,JJ;WalshJr,JV

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从蟾蜍(Bufo marinus)胃分离的内脏平滑肌细胞中记录两类钙通道的单通道电流:一类小电导通道(约11 pS)和一类大电导通道(约26 pS)。小电导通道存在于大多数贴片中,并产生缓慢失活电流(0 mV时t1/2 <250 ms)。开放的大电导通道可以明确地解决只有在存在的二氢吡啶钙+激动剂湾K 8644。发现了两种亚型的大电导通道-衰减速率非常慢(> 500 ms)和衰减速率较快(<100 ms)的通道。大电导通道类似于其他制剂的L-型Ca 2+通道。小电导通道并不明确地适合于其他现有类别(即,N或T)。单通道和宏观Ca 2+电流之间的对应关系进行了讨论。
Single-channel currents were recorded from two classes of Ca2+channels in visceral smooth muscle cells isolated from the stomach of the toad,Bufo marinus: a class of small-conductance channels (approximately 11 pS) and a class of large-conductance channels (approximately 26 pS). Small-conductance channels were present in a majority of patches and gave rise to a slowly inactivating current (t1/2≈ 250 ms at 0 mV). Openings of large-conductance channels could be unequivocally resolved only in the presence of the dihydropyridine Ca2+agonist Bay K 8644. Two subtypes of the large-conductance channels were found — those with a very slow rate of decay (> 500 ms) and those with a faster one (< 100ms). Large-conductance channels resemble L-type Ca2+channels of other preparations. Small-conductance channels do not fit unambiguously into the other existing categories (i.e., N or T). Correspondence between single-channel and macroscopic Ca2+currents is discussed.
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