Household Dust as a Repository of Chemical Accumulation: New Insights from a Comprehensive High-Resolution Mass Spectrometric Study.
Household Dust as a Repository of Chemical Accumulation: New Insights from a Comprehensive High-Resolution Mass Spectrometric Study.
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DOI：
10.1021/acs.est.7b05767
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发表时间：
2018-03-06
影响因子：
11.4
通讯作者：
Young TM
中科院分区：
文献类型：
--
作者：
Moschet C;Anumol T;Lew BM;Bennett DH;Young TM
家庭粉尘中的化学暴露对人类健康构成潜在风险，但迄今为止对其研究还不完整。大多数分析研究都集中在一个或几个化合物类上，通过LC-MS或GC-MS进行分析。然而，缺少对个别粉尘样本的全面调查。本研究综合运用目标、可疑和非目标筛选方法，利用LC-四极杆飞行时间(Q/TOF)和GC-Q/TOF对粉尘中的化学物质进行了综合表征。首先，优化了萃取方法，对LC-Q/TOF和GC-Q/TOF顺从性化合物的检测进行了优化，并成功地用100多种目标化合物进行了验证。用GC-Q/TOF进行非靶点筛选，通过光谱去卷积然后进行文库搜索。使用准确的质谱库进行LC-Q/TOF可疑筛选。最后，通过提取分子特征，获得串联的MS/MS谱图，并利用In-silo裂解软件工具进行化合物鉴定，进行LC-Q/TOF非靶标筛选。总共可以在38个粉尘样本中检测到258种化学物质；其中166种可以通过参考标准明确确认。其中许多有机氟化合物，如塑料可浸出7，9-Di-tert-butyl-1-oxaspiro(4，5)deca-6，9-diene-2，8-dione(CASRN：82304-66-3)和三种有机氟化合物正在引起新的关注，它们在粉尘中的存在被低估了。讨论了不同方法和分析仪器的优缺点。
Chemical exposure in household dust poses potential risks to human health but has been studied incompletely thus far. Most analytical studies have focused on one or several compound classes, with analysis performed by either LC-MS or GC-MS. However, a comprehensive investigation of individual dust samples is missing. The present study comprehensively characterizes chemicals in dust by applying a combination of target, suspect and non-target screening approaches using both LC-quadrupole time of flight (Q/TOF) and GC-Q/TOF. First, the extraction method was optimized to streamline detection of LC-Q/TOF and GC-Q/TOF amenable compounds and was successfully validated using over a hundred target compounds. Non-target screening with GC-Q/TOF was done by spectral deconvolution followed by a library search. Suspect screening by LC-Q/TOF was carried out using accurate mass spectral library. Finally, LC-Q/TOF non-target screening was carried out by extracting molecular features, acquiring tandem MS/MS spectra and performing compound identification using in-silico fragmentation software tools. In total, 258 chemicals could be detected in 38 dust samples; 166 of which could be unambiguously confirmed by a reference standard. Many of them, such as the plastic leachable 7,9-Di-tert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione (CASRN: 82304-66-3) and three organofluorine compounds are of emerging concern and their presence in dust has been underestimated. Advantages and drawbacks of the different approaches and analytical instruments are critically discussed.
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影响因子：
4.1
作者：
Gomez, M. J.;Gomez-Ramos, M. M.;Fernandez-Alba, A. R.
通讯作者：
Fernandez-Alba, A. R.
影响因子：
4.1
作者：
Negreira, N.;Rodriguez, I.;Cela, R.
通讯作者：
Cela, R.
影响因子：
11.4
作者：
Hollender, Juliane;Schymanski, Emma L.;Ferguson, P. Lee
通讯作者：
Ferguson, P. Lee
影响因子：
7.4
作者：
Megson, David;Robson, Matthew;Reiner, Eric J.
通讯作者：
Reiner, Eric J.
影响因子：
--
作者：
Corada-Fernandez, Carmen;Lara-Martin, Pablo A.;Gonzalez-Mazo, Eduardo
通讯作者：
Gonzalez-Mazo, Eduardo