Fundus autofluorescence lifetimes are increased in non‐proliferative diabetic retinopathy
Fundus autofluorescence lifetimes are increased in non‐proliferative diabetic retinopathy
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非增殖性糖尿病视网膜病变的眼底自发荧光寿命增加
DOI：
10.1111/aos.13174
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发表时间：
2016
影响因子：
3.4
通讯作者：
R. Augsten
中科院分区：
文献类型：
--
作者：
J. Schmidt;S. Peters;L. Sauer;D. Schweitzer;M. Klemm;R. Augsten
目的利用荧光终身成像眼底镜(Flio)对33例非增殖性糖尿病视网膜病变(NPDR)患者和28例正常对照的视网膜进行检测。由于糖尿病晶状体的荧光增强是已知的，34名患者和24名对照组的晶状体也进行了研究。在两个光谱通道(CH1：498-560 nm，CH2：560-720 nm)中检测到时间分辨衰减，并用一系列三个指数函数近似近似，分别得到寿命(τ1，τ2，τ3)、幅度(α1，α2，α3)及其幅度加权平均值(τm)。结果两个光谱通道的所有眼底寿命分量(τm，τ1-τ3)以及幅度α3在患者组和对照组之间均有显著差异。经Logistic回归模型分析，研究组和对照组的平均荧光寿命τ分别为90.09%和71.4%(曲线下面积为0.865)。患者组和对照组在晶体晶状体的平均荧光寿命τ分别为1587±326和1854±384pS，差异有统计学意义(P<0.006)。寿命的改变可能预示着晚期糖基化终末产物(AGEs)的积累，这使得对疾病的检测具有高的敏感性和特异性，可能具有诊断价值。
PurposeTo discriminate non‐proliferative diabetic retinopathy (NPDR) patients from healthy controls by fluorescence lifetime imaging ophthalmoscopy (FLIO).MethodsA prototype FLIO (Heidelberg‐Engineering, Heidelberg, Germany) was used to examine the retina of 33 patients and 28 controls. As increased fluorescence of the diabetic lens is known, the lenses of 34 patients and 24 controls were investigated as well. Time‐resolved decay was detected in two spectral channels (ch1: 498–560 nm, ch2: 560–720 nm) and approximated by a series of three exponential functions yielding in lifetimes (τ1,τ2,τ3), amplitudes (α1,α2,α3) and their amplitude‐weighted means (τm).ResultsSignificant differences between patients and controls were found for all fundus lifetime components (τm,τ1‐τ3) as for the amplitudeα3in both spectral channels. Channel 1 showed the largest differences: the average of mean fluorescence lifetimeτmin the macula was 259 ± 137 ps in the patients versus 147 ± 69 ps in the controls.A logistic regression model allowed discrimination between study and control group with a sensitivity of 90.09% and a specificity of 71.4% (area under the curve: 0.865).Significantly shorterτmin the patients group than in the control group was detected in channel 2 in the crystalline lens (1587 ± 326 ps versus 1854 ± 384 ps, p = 0.006).ConclusionsFundus Fluorescence lifetimes are significantly increased in NPDR while lens lifetimes are shorter in the patient group. Lifetime changes might be indicative for the accumulation of advanced glycation end products (AGEs) which enables detection of the disease with high sensitivity and specificity possibly bearing diagnostic merit.
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早发型和晚发型糖尿病中晶状体的自发荧光。
影响因子：
4.1
作者：
J M Sparrow;A. J. Bron;N. Brown;H A Neil
通讯作者：
H A Neil
与 1 型糖尿病和正常对照相比，2 型糖尿病的黄斑色素光学密度较低
DOI：
10.1097/iae.0000000000000551
发表时间：
2015
期刊：
Retina
影响因子：
--
作者：
Grainne Scanlon;P. Connell;Matthew Ratzlaff;Brittany Foerg;D. McCartney;Audrey Murphy;Karen O'Connor;J. Loughman
通讯作者：
J. Loughman
影响因子：
3.4
作者：
Field, Matthew G.;Yang, Dongli;Bian, Zong-Mei;Petty, Howard R.;Elner, Victor M.
通讯作者：
Elner, Victor M.
通过内源性荧光团的时间分辨自发荧光检测糖尿病患者眼底的早期代谢变化
影响因子：
3.1
作者：
Dietrich Schweitzer;M. Klemm;S. Quick;Lydia Deutsch;Susanne Jentsch;Martin Hammer;Jens Dawczynski;C. H. Kloos;U. A. Mueller
通讯作者：
U. A. Mueller
影响因子：
15.9
作者：
Lu, M;Kuroki, M;Adamis, AP
通讯作者：
Adamis, AP