N-trimethylchitosan/alginate layer-by-layer self assembly coatings act as "fungal repellents" to prevent biofilm formation on healthcare materials.
N-trimethylchitosan/alginate layer-by-layer self assembly coatings act as "fungal repellents" to prevent biofilm formation on healthcare materials.
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DOI：
10.1002/adhm.201400428
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发表时间：
2015-02-18
影响因子：
10
通讯作者：
Sun, Yuyu
中科院分区：
文献类型：
--
作者：
Jiang, Fuguang;Yeh, Chih-Ko;Wen, Jianchuan;Sun, Yuyu
医疗保健材料上的真菌生物膜形成是一个重要的临床问题，通常会导致难以治疗的医疗器械相关感染。开发了一种新型真菌驱避策略来控制真菌生物膜的形成。通过等离子体引发的接枝聚合，将甲基丙烯酸 (MAA) 磨碎到聚甲基丙烯酸甲酯 (PMMA) 基生物材料上。阳离子聚合物三甲基壳聚糖（TMC）是通过壳聚糖与碘甲烷反应合成的。海藻酸钠（SA）用作阴离子聚合物。 TMC/SA 多层膜通过逐层自组装涂覆在 MAA 接枝的 PMMA 上。 TMC/SA多层涂层显着降低真菌初始粘附，有效防止真菌生物膜形成。结论是表面的抗粘附特性归因于其亲水性，生物膜抑制作用归因于TMC的抗真菌活性以及TMC和SA的螯合功能，这可能起到了真菌驱避剂的作用。磷酸盐缓冲盐水 (PBS) 浸泡测试表明，多层涂层的生物膜调节效果稳定超过 4 周。此外，TMC/SA 多层涂层的存在提高了原始 PMMA 的生物相容性，为控制真菌生物膜的形成提供了一种简单而有效的策略。
Fungal biofilm formation on healthcare materials is a significant clinical concern, often leading to medical device related infections, which are difficult to treat. A novel fungal repellent strategy is developed to control fungal biofilm formation. Methylacrylic acid (MAA) is grated onto poly methyl methacrylate (PMMA)-based biomaterials via plasma initiated grafting polymerization. A cationic polymer, trimethylchitosan (TMC), is synthesized by reacting chitosan with methyl iodide. Sodium alginate (SA) is used as an anionic polymer. TMC/SA multilayers are coated onto the MAA-grafted PMMA via layer-by-layer self-assembly. The TMC/SA multilayer coatings significantly reduce fungal initial adhesion, and effectively prevent fungal biofilm formation. It is concluded that the anti-adhesive property of the surface is due to its hydrophilicity, and that the biofilm-inhibiting action is attributed to the antifungal activity of TMC as well as the chelating function of TMC and SA, which may have acted as fungal repellents. Phosphate buffered saline (PBS)-immersion tests show that the biofilm-modulating effect of the multilayer coatings is stable for more than 4 weeks. Furthermore, the presence of TMC/SA multilayer coatings improve the biocompatibility of the original PMMA, offering a simple, yet effective, strategy for controlling fungal biofilm-formation.
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影响因子：
7.6
作者：
RATANASATHIEN, S;WATAHA, JC;DENNISON, JB
通讯作者：
DENNISON, JB
DOI：
10.1038/nrmicro2711
发表时间：
2011-12-12
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.9
作者：
Ramage, G;vande Walle, K;López-Ribot, JL
通讯作者：
López-Ribot, JL
影响因子：
6.1
作者：
Belalia, Rachid;Grelier, Stephane;Coma, Veronique
通讯作者：
Coma, Veronique
影响因子：
4.5
作者：
Hariyadi, Dewi M.;Lin, Sharon Chien-Yu;Coombes, Allan G. A.
通讯作者：
Coombes, Allan G. A.