MicroRNA-resistant alleles of HOMEOBOX DOMAIN-2 modify inflorescence branching and increase grain protein content of wheat.
MicroRNA-resistant alleles of HOMEOBOX DOMAIN-2 modify inflorescence branching and increase grain protein content of wheat.
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DOI：
10.1126/sciadv.abn5907
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发表时间：
2022-05-13
期刊：
影响因子：
13.6
通讯作者：
中科院分区：
文献类型：
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植物和花序结构决定了作物的产量潜力。育种者利用花序结构的自然多样性来提高产量，诱导遗传变异可以提供进一步的收益。小麦是蛋白质和卡路里的重要来源；然而，人们对调控其花序发育的基因知之甚少。本研究在小麦a和D亚基因组上发现了一类同源结构域-亮氨酸拉链转录因子HOMEOBOX结构域-2 （HB-2）的半显性等位基因，该等位基因能产生更多的开花小穗并提高籽粒蛋白质含量。这些等位基因通过破坏在植物中具有保守作用的microRNA 165/166互补位点来增加HB-2的表达；较高的HB-2表达与籽粒发育过程中叶片和维管发育的改变以及花序氨基酸供应的增加有关。这些发现增加了我们对控制小麦花序发育的基因的认识，并为提高籽粒营养品质提供了途径。HB-2表达的增加改变了小麦的花序结构，改善了籽粒品质。
Plant and inflorescence architecture determine the yield potential of crops. Breeders have harnessed natural diversity for inflorescence architecture to improve yields, and induced genetic variation could provide further gains. Wheat is a vital source of protein and calories; however, little is known about the genes that regulate the development of its inflorescence. Here, we report the identification of semidominant alleles for a class III homeodomain-leucine zipper transcription factor, HOMEOBOX DOMAIN-2 (HB-2), on wheat A and D subgenomes, which generate more flower-bearing spikelets and enhance grain protein content. These alleles increase HB-2 expression by disrupting a microRNA 165/166 complementary site with conserved roles in plants; higher HB-2 expression is associated with modified leaf and vascular development and increased amino acid supply to the inflorescence during grain development. These findings enhance our understanding of genes that control wheat inflorescence development and introduce an approach to improve the nutritional quality of grain. Increased expression of HB-2 modifies inflorescence architecture and improves grain quality of wheat.
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影响因子：
46.9
作者：
Gaurav K;Arora S;Silva P;Sánchez-Martín J;Horsnell R;Gao L;Brar GS;Widrig V;John Raupp W;Singh N;Wu S;Kale SM;Chinoy C;Nicholson P;Quiroz-Chávez J;Simmonds J;Hayta S;Smedley MA;Harwood W;Pearce S;Gilbert D;Kangara N;Gardener C;Forner-Martínez M;Liu J;Yu G;Boden SA;Pascucci A;Ghosh S;Hafeez AN;O'Hara T;Waites J;Cheema J;Steuernagel B;Patpour M;Justesen AF;Liu S;Rudd JC;Avni R;Sharon A;Steiner B;Kirana RP;Buerstmayr H;Mehrabi AA;Nasyrova FY;Chayut N;Matny O;Steffenson BJ;Sandhu N;Chhuneja P;Lagudah E;Elkot AF;Tyrrell S;Bian X;Davey RP;Simonsen M;Schauser L;Tiwari VK;Randy Kutcher H;Hucl P;Li A;Liu DC;Mao L;Xu S;Brown-Guedira G;Faris J;Dvorak J;Luo MC;Krasileva K;Lux T;Artmeier S;Mayer KFX;Uauy C;Mascher M;Bentley AR;Keller B;Poland J;Wulff BBH
通讯作者：
Wulff BBH
影响因子：
7.4
作者：
Itoh, Jun-Ichi;Hibara, Ken-Ichiro;Nagato, Yasuo
通讯作者：
Nagato, Yasuo
影响因子：
7.4
作者：
Dobrovolskaya, Oxana;Pont, Caroline;Salse, Jerome
通讯作者：
Salse, Jerome
影响因子：
11.6
作者：
Gallavotti, Andrea;Malcomber, Simon;Schmidt, Robert J.
通讯作者：
Schmidt, Robert J.
DOI：
10.1242/dev.146399
发表时间：
2017-06-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Debernardi JM;Lin H;Chuck G;Faris JD;Dubcovsky J
通讯作者：
Dubcovsky J