Green cocoons in silkworm Bombyx mori resulting from the quercetin 5-O-glucosyltransferase of UGT86, is an evolved response to dietary toxins
Green cocoons in silkworm Bombyx mori resulting from the quercetin 5-O-glucosyltransferase of UGT86, is an evolved response to dietary toxins
复制标题
家蚕中的绿茧是由 UGT86 的槲皮素 5-O-葡萄糖基转移酶产生的，是对饮食毒素的进化反应
DOI：
10.1007/s11033-012-2437-7
复制
发表时间：
2012-12
影响因子：
2.8
通讯作者：
Xu, Shiqing
中科院分区：
文献类型：
--
作者：
Zhang, Dayan;Li, Juan;Yin, Weiming;Xu, Shiqing
UDP-葡萄糖基转移酶（UGT）的糖基化对于控制和消除内源性和外源性毒素至关重要。Bm-UGT 10286（UGT 86）是针对槲皮素的5-O位的UGT活性的唯一提供者，并且直接影响茧中绿色色素的形成。为了评估茧色是否进化为模拟目的，我们集中在Ugt 86的表达模式和酶底物的活性。Ugt 86不仅在消化管、丝腺等吸茧和积茧组织中有表达，而且在马氏管、脂肪体等解毒组织和性腺中也有表达。与在绿色茧品系中一样，Ugt 86在黄色和白色茧品系中清楚地表达。UGT 86融合蛋白在体外具有槲皮素代谢活性。然而，Ugt 86表达的5龄幼虫的丝腺中没有上调外源槲皮素。而在消化管和性腺中表达显著上调（P < 0.05）。在将幼虫暴露于芦丁（一种通常在植物中发现的昆虫抗性诱导剂和生长抑制剂）和20-羟基蜕皮激素（20 E）（一种昆虫内分泌和植物源激素）的实验中观察到类似的结果。而保幼激素Ⅲ处理组Ugt 86的表达几乎没有上调（P > 0.05）。HPLC结果表明，重组UGT 86蛋白与20 E在体外形成了一种新的物质，表明Ugt 86对20 E的作用与对植物源槲皮素的作用相似，可能是启动了对芦丁的解毒反应。结论：Ugt 86与蚕茧色素槲皮素的反应不是主动模拟生态发生的结果，而是UGT的解毒作用。
The glycosylation of UDP-glucosyltransferases (UGTs) is of great importance in the control and elimination of both endogenous and exogenous toxins. Bm-UGT10286 (UGT86) is the sole provider of UGT activity against the 5-O position of quercetin and directly influences the formation of green pigment in the Bombyx cocoon. To evaluate whether cocoon coloration evolved for mimetic purposes, we concentrated on the expression pattern of Ugt86 and the activities of the enzyme substrates. The expression of Ugt86 was not only detected in the cocoon absorbing and accumulating tissues such as the digestive tube and silk glands, but also in quantity in the detoxification tissues of the malpighian tubes and fat body, as well as in the gonads. As in the green cocoon strains, Ugt86 was clearly expressed in the yellow and white cocoon strains. In vitro, the fusion protein of UGT86 showed quercetin metabolic activity. Nevertheless, Ugt86 expression of 5th instar larvae was not up-regulated in the silk gland by exogenous quercetin. However, it was significantly up-regulated in the digestive tube and gonads (P < 0.05). A similar result was observed in experiments where larvae were exposed to rutin, an insect resistance inducer and growth inhibitor typically found in plants, and to 20-hydroxylecdysone (20E), an insect endocrine and plant source hormone. On the contrary, up-regulated Ugt86 expression was almost nil in larvae exposed to juvenile hormone III (P > 0.05). The results of HPLC revealed that a new substance was formed by mixing 20E with the recombinant UGT86 protein in vitro, indicating that the effect of Ugt86 on 20E was similar to that on exogenous quercetin derived from plant food, and that the effect probably initiated the detoxification reaction against rutin. The conclusion is that the reaction of Ugt86 on the silkworm cocoon pigment quercetin is not the result of active mimetic ecogenesis, but derives from the detoxification of UGTs.
登录
查看更多内容
影响因子：
2.9
作者：
REAL, MD;FERRE, J;CHAPA, FJ
通讯作者：
CHAPA, FJ
影响因子：
56.9
作者：
OREILLY, DR;MILLER, LK
通讯作者：
MILLER, LK
影响因子：
3.8
作者：
Hirayama, Chikara;Ono, Hiroshi;Nakamura, Masatoshi
通讯作者：
Nakamura, Masatoshi
影响因子：
6.1
作者：
BULL, DL;WHITTEN, CJ
通讯作者：
WHITTEN, CJ
DOI：
10.1073/pnas.0702860104
发表时间：
2007-05-22
影响因子：
11.1
作者：
Sakudoh, Takashi;Sezutsu, Hideki;Tsuchida, Kozo
通讯作者：
Tsuchida, Kozo