Single-Cell TCR Sequencing Reveals the Dynamics of T Cell Repertoire Profiling During Pneumocystis Infection.
Single-Cell TCR Sequencing Reveals the Dynamics of T Cell Repertoire Profiling During Pneumocystis Infection.
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单细胞 TCR 测序揭示肺孢子虫感染期间 T 细胞库谱分析的动态
DOI：
10.3389/fmicb.2021.637500
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发表时间：
2021
影响因子：
5.2
通讯作者：
Tong ZH
中科院分区：
文献类型：
--
作者：
Yang HQ;Wang YS;Zhai K;Tong ZH
T细胞应答在宿主对肺孢子虫的适应性免疫中起关键作用。然而，人类免疫缺陷病毒（HIV）阴性肺孢子虫肺炎（PCP）中T细胞免疫库的动态和多样性仍不清楚。在这项研究中，单细胞RNA和单细胞T细胞受体（TCR）测序应用于从肺孢子虫感染的小鼠肺组织中分选的细胞。结果表明，肺孢子虫感染后，克隆细胞主要由CD4+ T细胞组成，其特征是T细胞活化相关基因的高表达。与未感染的对照组相比，感染肺孢子虫的小鼠显示CD4+ T细胞中TCR多样性降低，CD8+ T细胞中TCR多样性增加。Th17细胞主要为克隆性CD4+ T细胞，具有组织驻留记忆样Th17细胞的表型。此外，肺孢子虫感染的小鼠表现出对TCRβ VDJ基因的偏倚使用。两者合计，我们的特点是转录组和TCR免疫谱的扩增T细胞克隆，这表明一个歪斜的TCR库肺孢子虫感染后。
T cell responses play critical roles in host adaptive immunity against Pneumocystis. However, the dynamics and diversity of the T cell immune repertoire in human immunodeficiency virus (HIV)-negative Pneumocystis pneumonia (PCP) remains unclear. In this study, single-cell RNA and single-cell T cell receptor (TCR) sequencing were applied to cells sorted from lung tissues of mice infected with Pneumocystis. Our findings demonstrated the clonal cells were mainly composed of CD4+ T cells in response to Pneumocystis, which were marked by highly expressed genes associated with T cell activation. Mice infected with Pneumocystis showed reduced TCR diversity in CD4+ T cells and increased diversity in CD8+ T cells compared with uninfected controls. Furthermore, Th17 cells were mostly clonal CD4+ T cells, which exhibited the phenotype of tissue-resident memory-like Th17 cells. In addition, Pneumocystis-infected mice showed biased usage of TCRβ VDJ genes. Taken together, we characterized the transcriptome and TCR immune repertoires profiles of expanded T cell clones, which demonstrate a skewed TCR repertoire after Pneumocystis infection.
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影响因子：
3.1
作者：
Kelly MN;Shellito JE
通讯作者：
Shellito JE
影响因子：
4.4
作者：
Wang P;Jin X;Zhou W;Luo M;Xu Z;Xu C;Li Y;Ma K;Cao H;Huang Y;Xue G;Jin S;Nie H;Jiang Q
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Jiang Q
影响因子：
9.3
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Klein, Allon M.
影响因子：
11.8
作者：
Roux A;Canet E;Valade S;Gangneux-Robert F;Hamane S;Lafabrie A;Maubon D;Debourgogne A;Le Gal S;Dalle F;Leterrier M;Toubas D;Pomares C;Bellanger AP;Bonhomme J;Berry A;Durand-Joly I;Magne D;Pons D;Hennequin C;Maury E;Roux P;Azoulay É
通讯作者：
Azoulay É
影响因子：
64.5
作者：
Azizi E;Carr AJ;Plitas G;Cornish AE;Konopacki C;Prabhakaran S;Nainys J;Wu K;Kiseliovas V;Setty M;Choi K;Fromme RM;Dao P;McKenney PT;Wasti RC;Kadaveru K;Mazutis L;Rudensky AY;Pe'er D
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Pe'er D