Screening and Identification of Exopolysaccharide-producing Bifidobacteria from Infant Gut

Screening and Identification of Exopolysaccharide-producing Bifidobacteria from Infant Gut
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婴儿肠道产胞外多糖双歧杆菌的筛选与鉴定

DOI:
10.19026/ajfst.7.1628
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发表时间:
2015
期刊:
Advance Journal of Food Science and Technology
影响因子:
--
通讯作者:
Xu Rihua
Xu Rihua
中科院分区:
其他
文献类型:
--
作者:
Li Jiaqi;Wang Jie;Guo Haitao;Xu Rihua

文献摘要

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乳酸菌产生的胞外多糖(EPS)具有多种生物活性和益生菌作用。然而,作为一种广为人知的益生菌,关于产生EPS的双歧杆菌的研究报道很少。本研究采用莫匹罗星锂盐改良培养基从婴儿粪便中分离到几株双歧杆菌,并对其产胞外多糖的性能进行了评价。在含2%蔗糖的MRS培养基上,通过乙醇沉淀法、透析法、苯酚-硫酸法等方法筛选出一株产EPS的双歧杆菌A19。通过生理生化特征、16S rDNA和TUF基因序列分析,将菌株A19鉴定为假卡氏双歧杆菌。该菌株是一株新的产胞外多糖的双歧杆菌,在添加2%蔗糖的MRS发酵液中,胞外多糖产量为55.546 mg/L。它可能成为微生态产品和食品发酵剂的潜在可用菌株。
Exopolysaccharide (EPS) produced from lactic acid bacteria has many bioactivities and the role of prebiotic as reported. However, being a well-knowen probiotic, there is little report about EPS-producing bifidobacteria. In this study, several bifidobacteria strains were isolated from baby feces with mupirocin lithium salt improved medium and evaluated for the production of EPS. Culturing strains with MRS medium containing 2% sucrose, one EPS-producing bifidobacteria strain A19 was screened by ethanol precipitation, dialysis, phenol- sulfuric acid method and other methods. By the physiological and biochemical characteristics, 16S rDNA and tuf gene sequence analysis, strain A19 was identified as Bifidobacterium pseudocatenulatum. It was a novel EPS- producing bifidobacteria and the EPS yield was 55.546 mg/L in MRS broth with 2% sucrose. It could be a potential available strain for microecologic products and food starters.
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