Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior.
Surface plasmon resonance fiber sensor for real-time and label-free monitoring of cellular behavior.
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DOI：
10.1016/j.bios.2014.01.018
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发表时间：
2014-06-15
影响因子：
12.6
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Shevchenko, Yanina;Camci-Unal, Gulden;Cuttica, Davide E.;Dokmeci, Mehmet R.;Albert, Jacques;Khademhosseini, Ali
本文报道了光纤生物传感器在细胞行为实时分析中的应用。我们的研究结果表明，由传统电信纤维制造的光纤传感器可以集成到传统的细胞培养设备中，用于实时和无标签监测细胞对化学刺激的反应。用于测量细胞响应的传感机制是基于光纤表面等离子体共振（SPR）的激发。在这项概念验证研究中，该传感器被用于研究多种不同刺激对细胞的影响——我们测试了胰蛋白酶、血清和叠氮化钠的作用。这些刺激诱导细胞脱离传感器表面，吸收血清和抑制细胞代谢。用alamar blue法、相衬法和荧光显微镜观察不同刺激物的作用。结果表明，纤维生物传感器可以成功地用于在引入刺激后的前30分钟内对细胞反应进行实时和无标记监测。此外，我们证明了光纤生物传感器可以很容易地再生以重复使用，证明了该平台是一种多功能且具有成本效益的传感工具。
This paper reports on the application of an optical fiber biosensor for real-time analysis of cellular behavior. Our findings illustrate that a fiber sensor manufactured from a traditional telecommunication fiber can be integrated into conventional cell culture equipment and used for real-time and label-free monitoring of cellular responses to chemical stimuli. The sensing mechanism used for the measurement of cellular responses is based on the excitation of Surface Plasmon Resonance (SPR) on the surface of the optical fiber. In this proof of concept study, the sensor was utilized to investigate the influence of a number of different stimuli on cells - we tested the effects of trypsin, serum and sodium azide. These stimuli induced detachment of cells from the sensor surface, uptake of serum and inhibition of cellular metabolism, accordingly. The effects of different stimuli were confirmed with alamar blue assay, phase contrast and fluorescence microscopy. The results indicated that the fiber biosensor can be successfully utilized for real-time and label-free monitoring of cellular response in the first 30 minutes following the introduction of a stimulus. Furthermore, we demonstrated that the optical fiber biosensors can be easily regenerated for repeated use, proving this platform as a versatile and cost-effective sensing tool.
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影响因子：
6.1
作者：
Pollet, J.;Delport, F.;Lammertyn, J.
通讯作者：
Lammertyn, J.
影响因子：
12.6
作者：
Francois, A.;Boehm, J.;Monro, T. M.
通讯作者：
Monro, T. M.
影响因子：
7.4
作者：
Shevchenko, Yanina;Francis, Tariq J.;Albert, Jacques
通讯作者：
Albert, Jacques
影响因子：
7.4
作者：
Ghaemmaghami, Amir M.;Hancock, Matthew J.;Harrington, Helen;Kaji, Hirokazu;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
7.2
作者：
Maltais, Jean-Sebastien;Denault, Jean-Bernard;Grandbois, Michel
通讯作者：
Grandbois, Michel