Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells.
Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells.
复制标题
DOI：
10.3390/molecules28073079
复制
发表时间：
2023-03-30
期刊：
影响因子：
--
通讯作者：
Furusawa Y
中科院分区：
文献类型：
--
作者：
Chudan S;Ishibashi R;Nishikawa M;Tabuchi Y;Nagai Y;Ikushiro S;Furusawa Y
小麦衍生的阿拉伯木聚糖（一种常见的膳食纤维）对健康的益处已经研究了几十年。然而，其对肠道微环境和炎症性肠病的影响尚不清楚。本研究的目的是了解小麦来源的阿拉伯木聚糖对肠道微生物群、结肠调节性T细胞（Tregs）和实验性结肠炎的影响。在本研究中，健康和慢性结肠炎模型小鼠喂食含有纤维素或小麦来源的阿拉伯木聚糖的食物2-6周，并进行后续分析。一项基于16s的粪便DNA宏基因组分析显示，在阿拉伯木聚糖喂养的小鼠中，含有丁酸盐产生菌和treg诱导菌的毛螺科细菌数量过多。与肠道菌群的变化一致，阿拉伯木聚糖喂养小鼠的粪便丁酸盐浓度和结肠Treg种群均升高。在慢性结肠炎的T细胞转移模型中，小麦来源的阿拉伯木聚糖改善了体重减轻和结肠组织炎症，这可能部分是由Treg诱导介导的。此外，在结肠炎模型中，小麦来源的阿拉伯木聚糖抑制了1型辅助性T细胞的TNFα产生。总之，通过改变肠道微环境，小麦衍生的阿拉伯木聚糖可能是一种很有希望的预防结肠炎的益生元。
The health benefits of wheat-derived arabinoxylan, a commonly consumed dietary fiber, have been studied for decades. However, its effect on the gut microenvironment and inflammatory bowel disease remains unclear. The objective of this study was to understand the effect of wheat-derived arabinoxylan on gut microbiota, colonic regulatory T cells (Tregs), and experimental colitis. In this study, healthy and chronic colitis model mice were fed chow containing cellulose or wheat-derived arabinoxylan for 2–6 weeks and subjected to subsequent analysis. A 16S-based metagenomic analysis of the fecal DNA revealed that Lachnospiraceae, comprising butyrate-producing and Treg-inducing bacteria, were overrepresented in arabinoxylan-fed mice. In line with the changes in the gut microbiota, both the fecal butyrate concentration and the colonic Treg population were elevated in the arabinoxylan-fed mice. In a T cell transfer model of chronic colitis, wheat-derived arabinoxylan ameliorated body weight loss and colonic tissue inflammation, which may, in part, be mediated by Treg induction. Moreover, wheat-derived arabinoxylan suppressed TNFα production from type 1 helper T cells in this colitis model. In conclusion, wheat-derived arabinoxylans, by altering the gut microenvironment, may be a promising prebiotic for the prevention of colitis.
登录
查看更多内容
影响因子：
12.2
作者：
Holscher HD
通讯作者：
Holscher HD
影响因子：
4.8
作者：
Brown, AJ;Goldsworthy, SM;Dowell, SJ
通讯作者：
Dowell, SJ
影响因子：
--
作者：
Rizzatti G;Lopetuso LR;Gibiino G;Binda C;Gasbarrini A
通讯作者：
Gasbarrini A
影响因子：
12.2
作者：
Müller M;Hermes GDA;Emanuel E C;Holst JJ;Zoetendal EG;Smidt H;Troost F;Schaap FG;Damink SO;Jocken JWE;Lenaerts K;Masclee AAM;Blaak EE
通讯作者：
Blaak EE
影响因子：
5.4
作者：
Jones JM
通讯作者：
Jones JM