Reporter proteins in whole-cell optical bioreporter detection systems, biosensor integrations, and biosensing applications.
Reporter proteins in whole-cell optical bioreporter detection systems, biosensor integrations, and biosensing applications.
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DOI:
10.3390/s91109147
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发表时间:
2009
期刊:
影响因子:
--
通讯作者:
Sayler GS
中科院分区:
文献类型:
--
作者:
Close DM;Ripp S;Sayler GS
Whole-cell, genetically modified bioreporters are designed to emit detectable signals in response to a target analyte or related group of analytes. When integrated with a transducer capable of measuring those signals, a biosensor results that acts as a self-contained analytical system useful in basic and applied environmental, medical, pharmacological, and agricultural sciences. Historically, these devices have focused on signaling proteins such as green fluorescent protein, aequorin, firefly luciferase, and/or bacterial luciferase. The biochemistry and genetic development of these sensor systems as well as the advantages, challenges, and common applications of each one will be discussed.
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