MicroRNA-Mediated Responses to Chromium Stress Provide Insight Into Tolerance Characteristics of Miscanthus sinensis.
MicroRNA-Mediated Responses to Chromium Stress Provide Insight Into Tolerance Characteristics of Miscanthus sinensis.
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DOI：
10.3389/fpls.2021.666117
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发表时间：
2021
影响因子：
5.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Nie G;Liao Z;Zhong M;Zhou J;Cai J;Liu A;Wang X;Zhang X
铬（Cr）是自然界中的一种重金属，释放到环境中对动物和植物都有潜在的毒性风险。然而，microRNA （miRNA）介导的对重金属Cr的反应调控在芒草中尚未被研究。本研究基于高通量miRNA测序，共鉴定出104个保守miRNA和158个非保守miRNA。其中，根中有45个差异表达mirna，叶中有13个差异表达mirna。分层聚类分析表明，这些mirna在特定组织中优先表达。根中有45种miRNA的833个差异表达靶基因，叶中有13种miRNA的280个差异表达靶基因。通过表达趋势分析，在根中获得5个显著富集模块，在叶中获得3个显著富集趋势块。基于候选基因注释和基因本体（GO）和京都基因与基因组百科全书（KEGG）功能分析，miR167a、novel_miR15和novel_miR22及其靶点可能参与了Cr的转运和螯合。此外，miR156a、miR164、miR396d和novel_miR155参与植物的生理生化代谢和Cr解毒。结果表明，mirna介导的对Cr处理的反应在中华绿芽孢杆菌中起关键作用，包括离子吸收、转运、积累和耐受特性。
Chromium (Cr) is a heavy metal in nature, which poses a potential risk to toxicity to both animals and plants when releasing into the environment. However, the regulation of microRNA (miRNA)-mediated response to heavy metal Cr has not been studied in Miscanthus sinensis. In this study, based on high-throughput miRNA sequencing, a total of 104 conserved miRNAs and 158 nonconserved miRNAs were identified. Among them, there were 45 differentially expressed miRNAs in roots and 13 differentially expressed miRNAs in leaves. The hierarchical clustering analysis showed that these miRNAs were preferentially expressed in a certain tissue. There were 833 differentially expressed target genes of 45 miRNAs in roots and 280 differentially expressed target genes of 13 miRNA in leaves. After expression trend analysis, five significantly enriched modules were obtained in roots, and three significantly enriched trend blocks in leaves. Based on the candidate gene annotation and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) function analysis, miR167a, novel_miR15, and novel_miR22 and their targets were potentially involved in Cr transportation and chelation. Besides, miR156a, miR164, miR396d, and novel_miR155 were identified as participating in the physiological and biochemical metabolisms and the detoxification of Cr of plants. The results demonstrated the critical role of miRNA-mediated responses to Cr treatment in M. sinensis, which involves ion uptake, transport, accumulation, and tolerance characteristics.
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/j.bbagrm.2011.05.001
发表时间：
2012-02
影响因子：
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