A novel mouse model of ischemic carotid artery disease.
A novel mouse model of ischemic carotid artery disease.
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DOI：
10.1371/journal.pone.0100257
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ihara M
中科院分区：
文献类型：
--
作者：
Hattori Y;Kitamura A;Nagatsuka K;Ihara M
随着时间的推移，颈动脉闭塞性疾病逐渐发展，最终导致脑梗死，死亡率很高。因此，已经开发了复制由颈动脉闭塞性疾病引起的脑梗死的动物模型来测试潜在的新治疗，其随后可以临床施用。成年C57 BL/6 J雄性小鼠接受Ameroid缩窄器（AC）放置以逐渐使双侧颈总动脉变窄。在几个时间点测量脑血流量（CBF）。在术后第7天和第28天，分析死后脑样品的缺血性变化。术后28天死亡率为58.8%。存活的AC小鼠在第28天显示CBF持续降低至基线水平的70%。大多数小鼠（75%）在灰质和白色物质中显示多发性脑梗死。未存活的小鼠在死亡前显示出低于基线水平的20-30%的临界CBF降低。AC在小鼠双侧颈总动脉上的应用可以提供由于颈动脉闭塞性疾病引起的严重脑血管功能不全的可靠模型，因此可以用于通过监测存活率、梗死形成和CBF特征来探索中风的药物干预。
Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential novel treatments, which could be subsequently administered clinically. Adult C57BL/6J male mice were subjected to ameroid constrictor (AC) placement to gradually narrow the bilateral common carotid arteries. Cerebral blood flow (CBF) was measured at several time points. At 7 and 28 days post-operation, post-mortem brain samples were analyzed for ischemic changes. The mortality rate was 58.8% at 28 days post-operation. Surviving mice with AC showed continuous reduction of CBF by up to 70% of the baseline level at 28 days. Most of the mice (75%) showed multiple cerebral infarctions in the gray and white matter. Non-surviving mice showed critical CBF reduction below 20–30% of the baseline level before death. The application of the AC on the bilateral common carotid arteries in mice could offer a reliable model of severe cerebrovascular insufficiency due to carotid artery occlusive disease and may thus be useful in exploring pharmacological intervention in stroke through monitoring survival rate, infarct formation, and CBF profile.
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影响因子：
2
作者：
Herrmann, M;Stern, M;Nitsch, C
通讯作者：
Nitsch, C
DOI：
10.1097/00004647-199805000-00012
发表时间：
1998-05-01
影响因子：
6.3
作者：
Kitagawa, K;Matsumoto, M;Yanagihara, T
通讯作者：
Yanagihara, T
DOI：
10.1097/01.wcb.0000122745.72175.d5
发表时间：
2004-07-01
影响因子：
6.3
作者：
Ayata, C;Dunn, AK;Moskowitz, MA
通讯作者：
Moskowitz, MA
影响因子：
9.9
作者：
Balestrini, Simona;Perozzi, Cecilia;Silvestrini, Mauro
通讯作者：
Silvestrini, Mauro
DOI：
10.1016/s0967-2109(98)00072-6
发表时间：
1998-12-01
期刊：
CARDIOVASCULAR SURGERY
影响因子：
--
作者：
AbuRahma, AF;Copeland, SE
通讯作者：
Copeland, SE