Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths.
Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths.
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DOI：
10.3390/ijms23010309
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发表时间：
2021-12-28
影响因子：
5.6
通讯作者：
Ohira A
中科院分区：
文献类型：
--
作者：
Kaidzu S;Okuno T;Tanito M;Ohira A
从功能和组织学上研究了可见光（从短波长到长波长）对视网膜的影响。Sprague-Dawley白化病大鼠左眼（6周龄，每个波长n = 6）暴露于7个窄带波长（中心波长：421、441、459、501、541、581和615 nm），带宽为16至29 nm（半带宽，±8-14.5 nm），使用具有带通滤波器的氙灯光源，在340和680 J/cm 2的视网膜辐射曝光下。未暴露的右眼作为对照。暴露7天后，记录闪光视网膜电图（ERG），并测量外核层（ONL）厚度。与未暴露的眼睛相比，暴露于460纳米或更短波长光的眼睛中的a波和b波ERG振幅显着降低。视神经乳头附近的ONL厚度也倾向于随着暴露于较短波长而减小。ERG振幅和ONL厚度的降低在两种辐射暴露下暴露于420 nm光的眼睛中最为突出。当波长相同时，辐射暴露量越高，损伤越强。与未暴露的眼睛相比，暴露于500 nm或更长波长光的眼睛的a波和b波没有显著降低。结果表明，在白化病大鼠中观察到的可见光引起的视网膜损伤依赖于暴露光的波长和能量水平。
The effects of visible light, from short to long wavelengths, on the retina were investigated functionally and histologically. The left eyes of Sprague–Dawley albino rats (6-weeks old, n = 6 for each wavelength) were exposed to seven narrow-band wavelengths (central wavelengths, 421, 441, 459, 501, 541, 581, and 615 nm) with bandwidths of 16 to 29 nm (half bandwidth, ±8–14.5 nm) using a xenon lamp source with bandpass filters at the retinal radiant exposures of 340 and 680 J/cm2. The right unexposed eyes served as controls. Seven days after exposure, flash electroretinograms (ERGs) were recorded, and the outer nuclear layer (ONL) thickness was measured. Compared to the unexposed eyes, significant reductions in the a- and b-wave ERG amplitudes were seen in eyes exposed to 460-nm or shorter wavelengths of light. The ONL thickness near the optic nerve head also tended to decrease with exposure to shorter wavelengths. The decreased ERG amplitudes and ONL thicknesses were most prominent in eyes exposed to 420-nm light at both radiant exposures. When the wavelengths were the same, the higher the amount of radiant exposure and the stronger the damage. Compared to the unexposed eyes, the a- and b-waves did not decrease significantly in eyes exposed to 500-nm or longer wavelength light. The results indicate that the retinal damage induced by visible light observed in albino rats depends on the wavelength and energy level of the exposed light.
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影响因子：
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作者：
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