Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
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DOI：
10.3389/fnut.2021.769341
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发表时间：
2021
影响因子：
5
通讯作者：
Vasu C
中科院分区：
文献类型：
--
作者：
Taylor HB;Vasu C
复杂的膳食多糖如β-葡聚糖因其抗炎特性而被广泛使用。我们之前报道过，成年小鼠口服酵母β-葡聚糖（YBG）可以通过调节肠道微生物群的结构和功能，帮助延迟1型糖尿病（T1 D）的发病并抑制肠道炎症。由于青少年年龄的特点是深刻改变不成熟的肠道微生物群，我们检查了口服治疗与YBG在非肥胖糖尿病（NOD）小鼠在这个年龄的影响。从15日龄开始每天口服给予YBG 7或30天的幼龄小鼠，检查肠道微生物群、免疫特征和T1 D发病率的变化。与对照组相比，接受YBG 30天但不是7天的小鼠在粪便微生物群的组成和多样性方面显示出相当大的变化。基于16 S rDNA序列的预测功能分析显示，在接受YBG 30天的小鼠中，聚糖生物合成和代谢、能量代谢和脂肪酸生物合成途径的过度表达。结肠的免疫表型显示向免疫调节和Th 17细胞因子倾斜，IL-10、IL-17和IL-21增加，TNF-α减少，但观察到一些促炎细胞因子（IL-1b、IFN-γ）增加。最重要的是，与对照组相比，接受YBG治疗30天的小鼠显示出显著抑制的胰岛炎和延迟的高血糖症发作。总的来说，这项研究表明，从糖尿病前期青少年年龄开始口服YBG可能对肠道微生物群和免疫功能产生积极的成熟变化，并可能导致那些易患T1 D的人延迟疾病发作。
Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties. We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota. Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age. Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence. Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls. Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days. Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed. Most importantly, mice that received YBG treatment for 30 days showed significantly suppressed insulitis and delayed onset of hyperglycemia compared to controls. Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
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影响因子：
11.1
作者：
Jian C;Carpén N;Helve O;de Vos WM;Korpela K;Salonen A
通讯作者：
Salonen A
影响因子：
4.2
作者：
Gudi, Radhika;Suber, Jada;Vasu, Chenthamarakshan
通讯作者：
Vasu, Chenthamarakshan
影响因子：
64.5
作者：
Chung H;Pamp SJ;Hill JA;Surana NK;Edelman SM;Troy EB;Reading NC;Villablanca EJ;Wang S;Mora JR;Umesaki Y;Mathis D;Benoist C;Relman DA;Kasper DL
通讯作者：
Kasper DL
影响因子：
6.1
作者：
Liu, Bo;Lin, Qinlu;Luo, Feijun
通讯作者：
Luo, Feijun
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y