N-glycome and N-glycoproteome of a hematophagous parasitic nematode Haemonchus.
N-glycome and N-glycoproteome of a hematophagous parasitic nematode Haemonchus.
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食血寄生线虫血矛线虫的 N-糖组和 N-糖蛋白质组
DOI：
10.1016/j.csbj.2021.04.038
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发表时间：
2021
影响因子：
6
通讯作者：
Hu M
中科院分区：
文献类型：
--
作者：
Wang C;Gao W;Yan S;Zhu XQ;Suo X;Liu X;Gupta N;Hu M
N-糖蛋白组。contortus公开了291种含有425个修饰位点的蛋白质。糖缀合物主要存在于寄生虫蠕虫的肠和性腺中。C1和M1肽酶家族的选定蛋白质在寄生虫的N-糖蛋白质组中富集。N-糖蛋白库与营养获取和免疫应答相关。我们的系统性N-糖基化分析将有助于疫苗开发的研究。N-糖基化是真核生物中蛋白质翻译后的重要修饰。对捻转血矛线虫（一种具有广泛地理分布的反刍动物吸血线虫）的初步研究表明，这种寄生虫含有具有排他性壳二糖修饰的N-聚糖。此外，几种免疫原性蛋白（例如，氨基-和金属-肽酶）已知在蠕虫中是N-糖基化的。然而，H. contortus尚未上市。在此，我们报告了291个N-糖基化蛋白，共425个修饰位点的寄生虫。其中，许多肽酶家族（例如，肽酶C1和M1），包括潜在的疫苗靶标。值得注意的是，富含聚糖的缀合物主要分布在蠕虫的肠和性腺中，因此隐藏在宿主的免疫系统之外。总的来说，这些数据提供了一个全面的地图集的N-糖基化在一个流行的寄生线虫，同时强调其意义的感染，免疫和预防。
N-glycoproteome of H. contortus discloses 291 proteins harboring 425 modification sites. Glycoconjugates occur predominantly in the intestine and gonads of the adult parasitic worms. Selected proteins of the C1 and M1 peptidase families are enriched in the parasite’s N-glycoproteome. A repertoire of N-glycoproteins is associated with the nutrient acquisition and immune response. Our systematic N-glycosylation profiling should facilitate research on vaccine development. N-glycosylation is a physiologically vital post-translational modification of proteins in eukaryotic organisms. Initial work on Haemonchus contortus – a blood-sucking nematode of ruminants with a broad geographical distribution – has shown that this parasite harbors N-glycans with exclusive chitobiose modifications. Besides, several immunogenic proteins (e.g., amino- and metallo-peptidases) are known to be N-glycosylated in adult worms. However, an informative atlas of N-glycosylation in H. contortus is not yet available. Herein, we report 291 N-glycosylated proteins with a total of 425 modification sites in the parasite. Among them, many peptidase families (e.g., peptidase C1 and M1) including potential vaccine targets were enriched. Notably, the glycan-rich conjugates are distributed primarily in the intestine and gonads of adult worms, and consequently hidden from the host’s immune system. Collectively, these data provide a comprehensive atlas of N-glycosylation in a prevalent parasitic nematode while underlining its significance for infection, immunity and prevention.
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影响因子：
4.3
作者：
Paschinger K;Wilson IB
通讯作者：
Wilson IB
影响因子：
5.8
作者：
Cheng, Alice;Grant, Charles E.;Bailey, Timothy L.
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Bailey, Timothy L.
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作者：
Loukas, A;Bethony, JM;Hotez, PJ
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12.3
作者：
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通讯作者：
Cotton JA
影响因子：
1.5
作者：
Hokke, Cornelis H.;van Diepen, Angela
通讯作者：
van Diepen, Angela