Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
Autoinducer-2 plays a crucial role in gut colonization and probiotic functionality of Bifidobacterium breve UCC2003.
复制标题
DOI：
10.1371/journal.pone.0098111
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Coenye T
中科院分区：
文献类型：
--
作者：
Christiaen SE;O'Connell Motherway M;Bottacini F;Lanigan N;Casey PG;Huys G;Nelis HJ;van Sinderen D;Coenye T
在本研究中，我们证明了短双歧杆菌UCC2003的LuxS参与了种间信号分子自动诱导因子-2(AI-2)的产生，该基因对小鼠胃肠道定植是必不可少的，同时它也参与了对秀丽线虫沙门氏菌感染的保护作用。我们证明了B.breve LuxS插入突变体比WT菌株对铁络合剂更敏感，并且这种敏感性在AI-2前体DPD的存在下可以部分逆转。此外，我们还发现，在体内条件下，铁饥饿诱导的基因簇中的几个基因在LuxS插入突变体中下调，并编码一个可能的铁摄取系统。B.breve UCC2003中这一簇的两个基因的突变使衍生的突变株对铁络合剂敏感，但缺乏向感染沙门氏菌的线虫提供肠道病原体保护的能力。由于在双歧杆菌属的所有受试成员中都存在一个具有功能的LuxS基因，我们得出结论，双歧杆菌在肠道定植和病原体保护方面运行着一个LuxS介导的系统，这与铁的获取有关。
In the present study we show that luxS of Bifidobacterium breve UCC2003 is involved in the production of the interspecies signaling molecule autoinducer-2 (AI-2), and that this gene is essential for gastrointestinal colonization of a murine host, while it is also involved in providing protection against Salmonella infection in Caenorhabditis elegans. We demonstrate that a B. breve luxS-insertion mutant is significantly more susceptible to iron chelators than the WT strain and that this sensitivity can be partially reverted in the presence of the AI-2 precursor DPD. Furthermore, we show that several genes of an iron starvation-induced gene cluster, which are downregulated in the luxS-insertion mutant and which encodes a presumed iron-uptake system, are transcriptionally upregulated under in vivo conditions. Mutation of two genes of this cluster in B. breve UCC2003 renders the derived mutant strains sensitive to iron chelators while deficient in their ability to confer gut pathogen protection to Salmonella-infected nematodes. Since a functional luxS gene is present in all tested members of the genus Bifidobacterium, we conclude that bifidobacteria operate a LuxS-mediated system for gut colonization and pathogen protection that is correlated with iron acquisition.
登录
查看更多内容
影响因子：
3.7
作者：
Cooper VS;Carlson WA;Lipuma JJ
通讯作者：
Lipuma JJ
影响因子：
9.8
作者：
Damman, Christopher J.;Miller, Samuel I.;Zisman, Timothy L.
通讯作者：
Zisman, Timothy L.
影响因子：
3.6
作者：
BASSLER, BL;WRIGHT, M;SILVERMAN, MR
通讯作者：
SILVERMAN, MR
影响因子：
5.8
作者：
de la Nava, JG;van Hijum, S;Trelles, O
通讯作者：
Trelles, O
影响因子：
4.9
作者：
Cronin, Michelle;Zomer, Aldert;van Sinderen, Douwe
通讯作者：
van Sinderen, Douwe