The Effect of Poplar PsnGS1.2 Overexpression on Growth, Secondary Cell Wall, and Fiber Characteristics in Tobacco.
The Effect of Poplar PsnGS1.2 Overexpression on Growth, Secondary Cell Wall, and Fiber Characteristics in Tobacco.
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杨树 PsnGS1.2 过表达对烟草生长、次生细胞壁和纤维特性的影响
DOI：
10.3389/fpls.2018.00009
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发表时间：
2018
影响因子：
5.6
通讯作者：
Wei Z
中科院分区：
文献类型：
--
作者：
Lu T;Liu L;Wei M;Liu Y;Qu Z;Yang C;Wei H;Wei Z
谷氨酰胺合成酶（GS 1）是氮代谢中催化谷氨酸和氨生成谷氨酰胺的关键酶。对GS1s基因在植物中的表达研究表明，GS1s基因的过量表达可以提高植物对氮素的利用率，促进植物的营养生长，改变木材的形成。本研究从小叶杨×黑杨中克隆了一个GS1基因，命名为PsnGS1.2。该基因在小黑杨根中表达量较高，在木质部、叶片和韧皮部中表达量较低，但可检测到。PsnGS1.2编码的蛋白主要位于细胞质中。PsnGS1.2基因在烟草中的过量表达导致了GS1活性和IAA含量的增加，氮同化作用增强，叶片增大，解剖结构改变。这些变化可能促进光合作用，生长和生物量生产力。值得注意的是，转PsnGS1.2基因烟草次生细胞壁和纤维特性发生了显著变化。这些变化与纤维发育、次生细胞壁成分生物合成、IAA生物合成、氨基酸运输和淀粉分解相关基因的表达水平改变一致。综上所述，我们的研究结果表明，PsnGS1. 2对转基因烟草氮素同化和代谢的催化作用，通过加速光合作用和IAA的合成，以及将碳通量转向合成更多的纤维素和半纤维素，对营养生长、叶片发育、次生细胞壁的形成和特性产生显著影响。
The glutamine synthetase (GS1) is a key enzyme that catalyzes the reaction of glutamate and ammonia to produce glutamine in the nitrogen (N) metabolism. Previous studies on GS1s in several plant species suggest that overexpression of GS1s can enhance N utilization, accelerate plant vegetative growth, and change wood formation. In this study, we isolated a GS1 gene, termed PsnGS1.2, from Populus simonii × Populus nigra. This gene was expressed at a higher level in roots, and relatively lower but detectable levels in xylem, leaves and phloem of P. simonii × P. nigra. The protein encoded by PsnGS1.2 is primarily located in the cytoplasm. Overexpression of PsnGS1.2 in tobacco led to the increased GS1 activity and IAA content, the augmented N assimilation, and the enlarged leaves with altered anatomical structures. These changes presumably promoted photosynthetic, growth, and biomass productivity. It was noteworthy that the secondary cell walls and fiber characteristics changed remarkably in PsnGS1.2 transgenic tobacco. These changes aligned well with the altered expression levels of the genes involved in fiber development, secondary cell wall component biosynthesis, IAA biosynthesis, amino acid transport, and starch breakdown. Taken together, the results from our study suggest that catalytic functions of PsnGS1.2 on N assimilation and metabolism in transgenic tobacco had significant effects on vegetative growth, leaf development, and secondary cell wall formation and properties through acceleration of photosynthesis and IAA biosynthesis, and redirection of carbon flux to synthesis of more cellulose and hemicellulose.
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影响因子：
13.8
作者：
Coleman, Heather D.;Canovas, Francisco M.;Mansfield, Shawn D.
通讯作者：
Mansfield, Shawn D.
Populus中的谷氨酰胺合成酶基因家族。
影响因子：
5.3
作者：
Castro-Rodríguez V;García-Gutiérrez A;Canales J;Avila C;Kirby EG;Cánovas FM
通讯作者：
Cánovas FM
影响因子：
5.1
作者：
Dubois, F;Brugiere, N;Hirel, B
通讯作者：
Hirel, B
影响因子：
6.4
作者：
Husted, S;Hebbern, CA;Schjoerring, JK
通讯作者：
Schjoerring, JK
影响因子：
7.4
作者：
Aspeborg, H;Schrader, J;Teeri, TT
通讯作者：
Teeri, TT