Cross-species infection of specific-pathogen-free pigs by a genotype 4 strain of human hepatitis E virus.
Cross-species infection of specific-pathogen-free pigs by a genotype 4 strain of human hepatitis E virus.
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DOI：
10.1002/jmv.21223
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发表时间：
2008-08
影响因子：
12.7
通讯作者：
Meng, X. J.
中科院分区：
文献类型：
--
作者：
Feagins, A. R.;Opriessnig, T.;Huang, Y. W.;Halbur, P. G.;Meng, X. J.
戊型肝炎病毒(HEV)是一种重要病原体。HEV的动物毒株猪HEV与人HEV有亲缘关系。3型猪戊型肝炎病毒感染人，3型人戊型肝炎病毒感染猪。猪和人的4型HEV毒株在基因上有亲缘关系，但尚不清楚4型人HEV是否能感染猪。本研究利用猪的生物测定法来确定基因4型人戊型肝炎病毒是否能感染猪。将15头4周龄无特定病原菌的猪分成3组，每组5头。第1组猪经静脉接种PBS缓冲液作为阴性对照，第2组猪与3型人戊型肝炎病毒相似(US-2株)，第3组猪与4型人戊型肝炎病毒相似(TW6196E株)。在接种后0天、7天、14天、21天、28天、35天、42天、49天和56天采集血清和粪便样本，并检测HEV感染的证据。所有猪在56dpi时进行尸检。不出所料，阴性对照猪仍为阴性。从HEV抗体、病毒血症和粪便病毒脱落的检测可以看出，接种了3型人HEV的阳性对照猪全部被感染。接种4型人戊型肝炎病毒的第3组的5头猪也全部被感染：从7~56dpi不同程度地检测到粪便病毒脱落和病毒血症，并在28dpi出现血清转换。结果表明，4型人HEV的宿主范围扩大，这一结果对了解HEV的自然历史和人畜共患病具有重要意义。
Hepatitis E virus (HEV) is an important pathogen. The animal strain of HEV, swine HEV, is related to human HEV. The genotype 3 swine HEV infected humans and genotype 3 human HEV infected pigs. The genotype 4 swine and human HEV strains are genetically related, but it is unknown whether genotype 4 human HEV can infect pigs. A swine bioassay was utilized in this study to determine whether genotype 4 human HEV can infect pigs. Fifteen, 4-week-old, specific-pathogen-free pigs were divided into 3 groups of 5 each. Group 1 pigs were each inoculated intravenously with PBS buffer as negative controls, group 2 pigs similarly with genotype 3 human HEV (strain US-2), and group 3 pigs similarly with genotype 4 human HEV (strain TW6196E). Serum and fecal samples were collected at 0, 7, 14, 21, 28, 35, 42, 49, and 56 days postinoculation (dpi) and tested for evidence of HEV infection. All pigs were necropsied at 56 dpi. As expected, the negative control pigs remained negative. The positive control pigs inoculated with genotype 3 human HEV all became infected as evidenced by detection of HEV antibodies, viremia and fecal virus shedding. All five pigs in group 3 inoculated with genotype 4 human HEV also became infected: fecal virus shedding and viremia were detected variably from 7 to 56 dpi, and seroconversion occurred by 28 dpi. The data indicated that genotype 4 human HEV has an expanded host range, and the results have important implications for understanding the natural history and zoonosis of HEV.
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影响因子：
3.8
作者：
Haqshenas, G;Shivaprasad, HL;Meng, XJ
通讯作者：
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影响因子：
9.4
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3.8
作者：
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影响因子：
5.4
作者：
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通讯作者：
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2.2
作者：
Guo, Hailong;Zhou, E. M.;Meng, X. J.
通讯作者：
Meng, X. J.