Full Genomic Analysis of New Variants of Porcine Reproductive and Respiratory Syndrome Virus Revealed Multiple Recombination Events Between Different Lineages and Sublineages.
Full Genomic Analysis of New Variants of Porcine Reproductive and Respiratory Syndrome Virus Revealed Multiple Recombination Events Between Different Lineages and Sublineages.
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猪繁殖与呼吸综合征病毒新变种的全基因组分析揭示了不同谱系和亚谱系之间的多重重组事件
DOI：
10.3389/fvets.2020.00603
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发表时间：
2020
影响因子：
3.2
通讯作者：
Wei Z
中科院分区：
文献类型：
--
作者：
Wang J;Lin S;Quan D;Wang H;Huang J;Wang Y;Ren T;Ouyang K;Chen Y;Huang W;Luo T;Wei Z
猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus，PRRSV）对我国养猪业造成了严重的危害，对其遗传多样性进行监测对于了解其流行病学和进化具有重要意义。在这里，我们确定了两个PRRSV株，GXYL 1403和GXNN 1839的全基因组序列。比较，系统发育和重组检测程序分析表明，这两个分离株是重组株与大片段的nsp 2氨基酸缺失。与VR 2332相比，GXYL 1403在nsp 2中具有124个氨基酸的独特缺失区，GXNN 1839在nsp 2中含有131个氨基酸的缺失。对全长序列的进一步分析表明，GXYL 1403是亚系8.1（CH-1a样）和8.3（JXA 1样）之间的天然重组体。GXYL 1403的重组位点位于nsp 9-nsp 12（8961 nt-11181 nt）。GXNN 1839是谱系5（VR-2332样）和谱系1（NADC 30样）菌株之间的天然重组体。重组事件发生在GXNN 1839基因组中的nsp 9（7872 nt-8162 nt）和ORF 2（12587 nt-13282 nt）中。这些结果提供了新的证据表明，在环境中循环的PRRSV毒株已经经历了重组之间的不同谱系或亚系的现场菌株，这些增加了我们的理解的RNA组合事件发生在PRRSV。
Porcine reproductive and respiratory syndrome virus (PRRSV) has had a devastating impact on the pig industry in China, and monitoring its genetic diversity is important for epidemiological surveillance and understanding its evolution. Here, we determine the complete genome sequences of two PRRSV strains, GXYL1403 and GXNN1839. Comparative, phylogenetic, and recombination detection program analyses show that the two isolates are recombinant strains with large-fragment amino acid deletions in nsp2. GXYL1403 possesses a unique deletion region of 124 amino acids in nsp2, and GXNN1839 contains a deletion of 131 amino acids in nsp2 as compared with VR2332. Further analysis of the full-length sequence suggests that GXYL1403 is a natural recombinant between sublineages 8.1 (CH-1a like) and 8.3 (JXA1-like). The recombination site of GXYL1403 is located in nsp9–nsp12 (8961nt−11181nt). GXNN1839 is a natural recombinant between the lineage 5 (VR-2332-like) and lineage 1 (NADC30-like) strains. The recombination events occurred in nsp9 (7872nt-8162nt) and in ORF2 (12587nt−13282nt) in the genome of GXNN1839. These results provide new evidence that PRRSV strains circulating in the environment have undergone recombination among the different lineages or sublineages of field strains, and these add to our understanding of RNA combination events that occur in PRRSV.
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中国不同重组猪繁殖与呼吸综合征病毒的出现
影响因子：
4.6
作者：
Liu Y;Li J;Yang J;Zeng H;Guo L;Ren S;Sun W;Chen Z;Cong X;Shi J;Chen L;Du Y;Li J;Wang J;Wu J;Yu J
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Yu J
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影响因子：
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作者：
Sun, Yan-Kuo;Li, Qi;Wang, Heng
通讯作者：
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影响因子：
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Li, C.;Zhuang, J.;Tian, K.
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