The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors.
The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors.
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犬自发性血管内皮细胞瘤中过氧化还蛋白1和2的免疫组织化学检测
DOI：
10.1292/jvms.22-0102
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发表时间：
2022-07-01
影响因子：
1.2
通讯作者：
Sakai, Hiroki
中科院分区：
文献类型：
--
作者：
Otsuka, Narumi;Ishimaru, Kairi;Murakami, Mami;Goto, Minami;Hirata, Akihiro;Sakai, Hiroki
过氧化还蛋白(PRDX)是一个抗氧化酶家族，有六种异构体(PRDX1-6)。PRDXs的主要功能是通过将过氧化氢等活性氧物种还原为H2O来降低细胞的氧化应激。近年来，研究发现PRDX在人类多种恶性肿瘤中均有过表达，并参与肿瘤的发生、增殖和转移。然而，关于PRDXs在动物肿瘤中的表达的研究还很有限。因此，在本研究中，我们用免疫组织化学方法检测了PRDX1和2在自发性犬血管肉瘤(HSA)和血管瘤(HA)中的表达，以及在选定的正常组织和肉芽组织(包括新生血管)中的表达。虽然有一些例外，但PRDX1和PRDX2在正常犬组织中的免疫定位与在人类、大鼠或小鼠中的相似。肉芽组织中，新生血管内皮细胞PRDX1和PRDX2呈强阳性表达，而成熟血管中静止的内皮细胞呈阴性表达。PRDX1和PRDX2在HSA中的表达均显著高于HA。PRDX1和PRDX2在不同亚型和不同原发部位HSA中的表达差异无统计学意义。这些结果提示，PRDX1和PRDX2可能参与肉芽组织中内皮细胞的血管生成表型以及在恶性内皮细胞瘤中的行为。
Peroxiredoxin (PRDX) is an antioxidant enzyme family with six isoforms (PRDX1–6). The main function of PRDXs is to decrease cellular oxidative stress by reducing reactive oxygen species, such as hydrogen peroxide, to H2O. Recently, it has been reported that PRDXs are overexpressed in various malignant tumors in humans, and are involved in the development, proliferation, and metastasis of tumors. However, studies on the expression of PRDXs in tumors of animals are limited. Therefore, in the present study, we immunohistochemically investigated the expression of PRDX1 and 2 in spontaneous canine hemangiosarcoma (HSA) and hemangioma (HA), as well as in selected normal tissue and granulation tissue, including newly formed blood vessels. Although there were some exceptions, immunolocalization of PRDX1 and 2 in normal canine tissues was similar to those in humans, rats, or mice. In granulation tissue, angiogenic endothelial cells were strongly positive for PRDX1 and 2, whereas quiescent endothelial cells in mature vessels were negative. Both PRDX1 and 2 were significantly highly expressed in HSA compared to HA. There were no significant differences in the expression of PRDX1 and 2 among the subtypes and primary sites of HSA. These results suggest that PRDX1 and 2 may be involved in the angiogenic phenotypes of endothelial cells in granulation tissue as well as in the behavior in the malignant endothelial tumors.
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影响因子：
6.5
作者：
Lee, SC;Chae, HZ;Kim, YP
通讯作者：
Kim, YP
影响因子：
2.6
作者：
Murai A;Asa SA;Kodama A;Hirata A;Yanai T;Sakai H
通讯作者：
Sakai H
影响因子：
0.8
作者：
Anwar, Sh.;Yanai, T.;Sakai, H.
通讯作者：
Sakai, H.
影响因子：
3.9
作者：
Lu, Ermei;Hu, Xiaoli;Zhu, Xueqiong
通讯作者：
Zhu, Xueqiong
影响因子：
2.5
作者：
Mizusawa, H;Ishii, T;Bannai, S
通讯作者：
Bannai, S