In Vitro Strategies to Vascularize 3D Physiologically Relevant Models.
In Vitro Strategies to Vascularize 3D Physiologically Relevant Models.
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DOI：
10.1002/advs.202100798
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Simon-Yarza T
中科院分区：
文献类型：
--
作者：
Dellaquila A;Le Bao C;Letourneur D;Simon-Yarza T
3D模型的血管化是组织工程的主要挑战，也是其临床和工业应用的关键先决条件。使用由专用材料构建的预血管化模型可以解决一些实际限制，例如生物构建物在宿主组织内的次优整合，并将提供更多体内类似的灌注组织和器官特异性平台。在过去的十年中，血管化的生理相关的3D构建体的制造已经尝试了许多组织工程的策略，这在这里被分类在微流体技术，3D共培养模型，即，球状体和类器官，和生物纤维素。在这篇综述中，讨论了血管前技术的最新进展以及越来越多地使用天然和合成材料来构建生理器官特异性模型。目前的缺点，每种技术，未来的前景，和翻译的血管化组织结构向临床，制药领域，和工业也提出。通过结合互补策略，这些模型有望在不久的将来成功用于再生医学和药物开发。本文就三维组织工程模型的血管化策略作一综述。体外血管网络的整合是实现具有生理相关性的组织构建的基础。如今，已经开发了许多方法来使组织和器官模型血管化，主要是通过使用微流体技术，3D细胞培养和生物打印。最近的进展和这些策略的组合正在促进向临床和工业领域的快速转化。
Vascularization of 3D models represents a major challenge of tissue engineering and a key prerequisite for their clinical and industrial application. The use of prevascularized models built from dedicated materials could solve some of the actual limitations, such as suboptimal integration of the bioconstructs within the host tissue, and would provide more in vivo‐like perfusable tissue and organ‐specific platforms. In the last decade, the fabrication of vascularized physiologically relevant 3D constructs has been attempted by numerous tissue engineering strategies, which are classified here in microfluidic technology, 3D coculture models, namely, spheroids and organoids, and biofabrication. In this review, the recent advancements in prevascularization techniques and the increasing use of natural and synthetic materials to build physiological organ‐specific models are discussed. Current drawbacks of each technology, future perspectives, and translation of vascularized tissue constructs toward clinics, pharmaceutical field, and industry are also presented. By combining complementary strategies, these models are envisioned to be successfully used for regenerative medicine and drug development in a near future. Vascularization strategies of 3D tissue engineered models are reviewed in this article. The integration of a vascular network in vitro is fundamental to achieve tissue constructs with physiological relevance. Nowadays, numerous approaches have been developed to vascularize tissue and organ models, mainly by using microfluidic technology, 3D cell culture, and bioprinting. Recent advances and the combination of these strategies are promoting a rapid translation toward the clinical and industrials fields.
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