Clinical relevance of the in situ assay for HBV DNA: a cross-sectional study in patients with chronic hepatitis B.
Clinical relevance of the in situ assay for HBV DNA: a cross-sectional study in patients with chronic hepatitis B.
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HBV DNA 原位检测的临床相关性：慢性乙型肝炎患者的横断面研究
DOI：
10.1136/jclinpath-2020-206440
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发表时间：
2020-12
影响因子：
3.4
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Liu D;Xu T;Shi B;Lu W;Zheng Y;Feng Y;Yuan Z;Zhang X;Zhang Z
目的我们以前的报告中慢性B型肝炎（CH B）患者肝切片中HBV DNA的可视化发现了病毒抗原和核酸的镶嵌分布。在这里，我们的目的是进一步探讨原位杂交（ISH）检测HBV DNA的临床实用性。方法对313例初治CHB患者进行HBV DNA沿着原位杂交、HBsAg、HBcAg免疫组化及常规组织病理学检查。血清HBcrAg和HBcAb滴度也进行了测量，除了基本的生化和病毒学参数。结果原位杂交检测HBVDNA、HBsAg和HBcAg的阳性率分别为95.2%、97.1%和42.8%。HBV DNA的染色模式与HBsAg有显著差异。肝内HBV DNA与病毒载量、HBcrAg和HBsAg滴度高度相关。在HBeAg阴性患者中，较高的肝内HBV DNA与肝脏炎症和纤维化的组织学证据相关，而在HBeAg阳性患者中没有观察到这种趋势。最后，开发了结合HBV DNA、HBsAg和胶原纤维检测的三重染色方案，以更好地评价疾病进展背景下的病毒复制和抗原表达。结论原位杂交检测HBV DNA能反映肝内病毒复制的活力。它是病毒抗原常规IHC检测的补充，也与肝脏疾病的组织病理学进展有关。HBV DNA原位杂交技术的应用有助于更好地评价慢性乙型肝炎患者的病毒学和病理学状态。
Aims The visualisation of HBV DNA in liver sections of patients with chronic hepatitis B (CHB) in our previous report uncovered a mosaic distribution of viral antigens and nucleic acids. Here we aim to further explore the clinical utility of the in situ hybridisation (ISH) assay for HBV DNA. Method ISH of HBV DNA along with immunohistochemistry (IHC) of HBsAg, HBcAg and routine histopathology analysis was performed in 313 treatment-naive patients with CHB. Serum HBcrAg and HBcAb titre were also measured in addition to basic biochemical and virological parameters. Results The ISH of HBV DNA, HBsAg and HBcAg showed 95.2%, 97.1% and 42.8% positive rate, respectively. The staining pattern of HBV DNA differs significantly with that of HBsAg. Intrahepatic HBV DNA exhibited high-level of correlations with viral load, HBcrAg and HBsAg titre. In HBeAg-negative patients, higher intrahepatic HBV DNA is associated with histological evidence of liver inflammation and fibrosis, whereas no such trend was observed in HBeAg-positive patients. Finally, a triple staining protocol that combined the detection of HBV DNA, HBsAg and collagen fibre was developed to enable better evaluation of viral replication and antigen expression in the context of disease progression. Conclusions The ISH assay for HBV DNA reflects the vigour of intrahepatic viral replication. It is complementary to the routine IHC assay for viral antigens and also related to the histopathological progression of liver diseases. The application of the HBV DNA ISH assay may help a better evaluation of virological and pathological condition of patients with CHB.
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乙型肝炎病毒感染自然史中乙型肝炎核心抗体水平的定量。
影响因子：
14.2
作者：
Song, L. -W.;Liu, P. -G.;Xia, N. -S.
通讯作者：
Xia, N. -S.
影响因子：
14.2
作者：
van Campenhout, M. J. H.;Brouwer, W. P.;Janssen, H. L. A.
通讯作者：
Janssen, H. L. A.
影响因子：
3.1
作者：
Zhang, Zhan-Qing;Lu, Wei;Feng, Yan-Ling
通讯作者：
Feng, Yan-Ling
影响因子：
6.6
作者：
Sarin SK;Kumar M;Lau GK;Abbas Z;Chan HL;Chen CJ;Chen DS;Chen HL;Chen PJ;Chien RN;Dokmeci AK;Gane E;Hou JL;Jafri W;Jia J;Kim JH;Lai CL;Lee HC;Lim SG;Liu CJ;Locarnini S;Al Mahtab M;Mohamed R;Omata M;Park J;Piratvisuth T;Sharma BC;Sollano J;Wang FS;Wei L;Yuen MF;Zheng SS;Kao JH
通讯作者：
Kao JH
影响因子：
2.4
作者：
Zhang ZQ;Zhang XN;Lu W;Wang YB;Weng QC;Feng YL
通讯作者：
Feng YL