Integrating Transcriptome and Coexpression Network Analyses to Characterize Salicylic Acid- and Jasmonic Acid-Related Genes in Tolerant Poplars Infected with Rust.
Integrating Transcriptome and Coexpression Network Analyses to Characterize Salicylic Acid- and Jasmonic Acid-Related Genes in Tolerant Poplars Infected with Rust.
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整合转录组和共表达网络分析来表征感染锈病的耐性杨树中的水杨酸和茉莉酸相关基因
DOI：
10.3390/ijms22095001
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发表时间：
2021-05-08
影响因子：
5.6
通讯作者：
Wang F
中科院分区：
文献类型：
--
作者：
Chen Q;Zhang R;Li D;Wang F
杨栅锈菌（Melampsora larici - populina）在世界范围内引起严重的杨树叶片病害——锈病。水杨酸（SA）和茉莉酸（JA）是与植物防御反应相关的重要植物激素。为了研究参与杨树与锈菌相互作用的水杨酸和茉莉酸相关基因的转录组图谱，本研究选取了两种耐锈病杨树和一种不耐锈病杨树。采用加权基因共表达网络分析（WGCNA）来表征在感染杨栅锈菌强毒力E4小种后转录组图谱以及水杨酸和茉莉酸含量的变化。在对杨栅锈菌E4小种感染的响应中，耐病症状与水杨酸和茉莉酸生物合成相关基因的表达、水杨酸和茉莉酸的水平以及水杨酸和茉莉酸下游防御相关基因的表达相关。耐病杨树能够通过及时激活或抑制水杨酸或茉莉酸途径迅速调节防御反应的发生，包括调节与程序性细胞死亡相关基因的表达，如 Kunitz型胰蛋白酶抑制剂（KTI），以限制E4的生长并保护自身。WGCNA表明KTI可能受细胞色素P450家族（CYP）基因的调控。一些CYPs在茉莉酸和水杨酸相关途径中都应发挥重要作用。相反，在不耐病杨树中，早期通过提高茉莉酸水平对水杨酸相关防御信号的抑制导致大量植物 - 病原体相互作用相关和信号相关基因持续受到抑制，包括NBS - LRRs、EDS1、NDR1、WRKYs和PRs。因此，及时激活或抑制水杨酸或茉莉酸途径是耐病杨树和不耐病杨树之间的关键差异。
Melampsora larici-populina causes serious poplar foliar diseases called rust worldwide. Salicylic acid (SA) and jasmonic acid (JA) are important phytohormones that are related to plant defence responses. To investigate the transcriptome profiles of SA- and JA-related genes involved in poplar rust interaction, two tolerant poplars and one intolerant poplar were selected for this study. Weighted gene coexpression network analysis (WGCNA) was applied to characterize the changes in the transcriptome profiles and contents of SA and JA after infection with the virulent E4 race of M. larici-populina. In response to infection with the E4 race of M. larici-populina, tolerant symptoms were correlated with the expression of genes related to SA and JA biosynthesis, the levels of SA and JA, and the expression of defence-related genes downstream of SA and JA. Tolerant poplars could promptly regulate the occurrence of defence responses by activating or inhibiting SA or JA pathways in a timely manner, including regulating the expression of genes related to programmed cell death, such as Kunitz-type trypsin inhibitor (KTI), to limit the growth of E4 and protect themselves. WGCNA suggested that KTI might be regulated by a Cytochrome P450 family (CYP) gene. Some CYPs should play an important role in both JA- and SA-related pathways. In contrast, in intolerant poplar, the inhibition of SA-related defence signalling through increasing JA levels in the early stage led to continued inhibition of a large number of plant–pathogen interaction-related and signalling-related genes, including NBS-LRRs, EDS1, NDR1, WRKYs, and PRs. Therefore, timely activation or inhibition of the SA or JA pathways is the key difference between tolerant and intolerant poplars.
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影响因子：
4.3
作者：
Dean, JV;Mohammed, LA;Fitzpatrick, T
通讯作者：
Fitzpatrick, T
影响因子：
7.4
作者：
Broekgaarden, Colette;Caarls, Lotte;Van Wees, Saskia C. M.
通讯作者：
Van Wees, Saskia C. M.
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
影响因子：
3.5
作者：
Fitzgerald, HA;Chern, MS;Ronald, PC
通讯作者：
Ronald, PC
影响因子：
11.4
作者：
Feys, BJ;Moisan, LJ;Parker, JE
通讯作者：
Parker, JE