Tracking tonic dopamine levels in vivo using multiple cyclic square wave voltammetry.
Tracking tonic dopamine levels in vivo using multiple cyclic square wave voltammetry.
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DOI：
10.1016/j.bios.2018.08.034
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发表时间：
2018-12-15
影响因子：
12.6
通讯作者：
Jang DP
中科院分区：
文献类型：
--
作者：
Oh Y;Heien ML;Park C;Kang YM;Kim J;Boschen SL;Shin H;Cho HU;Blaha CD;Bennet KE;Lee HK;Jung SJ;Kim IY;Lee KH;Jang DP
二十多年来，快速扫描循环伏安法（FSCV）一直是近实时监测体内多巴胺释放的可靠分析方法。然而，当代FSCV技术仅限于测量由电刺激诱发或由行为显著刺激的呈现引起的多巴胺释放的快速（秒级，即阶段性）变化，而不是多巴胺的紧张性细胞外水平（即基础浓度）的较慢变化。这是因为FSCV本质上是一种差分方法，需要减去多巴胺的刺激前紧张水平，以测量相对于归零基线的相位变化。在这里，我们描述了一种新的伏安法技术，多循环方波伏安法（M-CSWV）的开发和应用，分析定量的滋补多巴胺浓度在体内具有相对较高的时间分辨率（10秒）。M-CSWV通过产生二维伏安图来丰富电化学信息，所述二维伏安图能够实现对抗坏血酸和3，4-二羟基苯乙酸（DOPAC）的高灵敏度（检测限，0.17 nM）和选择性，包括pH的变化。使用M-CSWV，在乌拉坦麻醉大鼠的纹状体中测定120 ± 18 nM（n = 7只大鼠，± SEM）的紧张性多巴胺浓度。通过选择性抑制多巴胺再摄取来升高多巴胺和通过抑制单胺氧化酶来降低DOPAC的药理学治疗支持体内多巴胺的选择性检测。总的来说，M-CSWV提供了一种新的伏安法技术来量化水平和监测大脑中紧张性多巴胺浓度的变化，以进一步了解多巴胺在正常行为和神经精神疾病中的作用。
For over two decades, fast-scan cyclic voltammetry (FSCV) has served as a reliable analytical method for monitoring dopamine release in near real-time in vivo. However, contemporary FSCV techniques have been limited to measure only rapid (on the order of seconds, i.e. phasic) changes in dopamine release evoked by either electrical stimulation or elicited by presentation of behaviorally salient stimuli, and not slower changes in the tonic extracellular levels of dopamine (i.e. basal concentrations). This is because FSCV is inherently a differential method that requires subtraction of prestimulation tonic levels of dopamine to measure phasic changes relative to a zeroed baseline. Here, we describe the development and application of a novel voltammetric technique, multiple cyclic square wave voltammetry (M-CSWV), for analytical quantification of tonic dopamine concentrations in vivo with relatively high temporal resolution (10 s). M-CSWV enriches the electrochemical information by generating two dimensional voltammograms which enable high sensitivity (limit of detection, 0.17 nM) and selectivity against ascorbic acid, and 3,4-dihydroxyphenylacetic acid (DOPAC), including changes in pH. Using M-CSWV, a tonic dopamine concentration of 120 ± 18 nM (n = 7 rats, ± SEM) was determined in the striatum of urethane anethetized rats. Pharmacological treatments to elevate dopamine by selectively inhibiting dopamine reuptake and to reduce DOPAC by inhibition of monoamine oxidase supported the selective detection of dopamine in vivo. Overall, M-CSWV offers a novel voltammetric technique to quantify levels and monitor changes in tonic dopamine concentrations in the brain to further our understanding of the role of dopamine in normal behavior and neuropsychiatric disorders.
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影响因子：
4.5
作者：
Dengler, Adam K.;McCarty, Gregory S.
通讯作者：
McCarty, Gregory S.
DOI：
10.1039/b807563h
发表时间：
2009-01
期刊：
The Analyst
影响因子：
--
作者：
Huffman ML;Venton BJ
通讯作者：
Venton BJ
影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
7.4
作者：
Bath, BD;Michael, DJ;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
5
作者：
Burrell, Mark H.;Atcherley, Christopher W.;Lipski, Janusz
通讯作者：
Lipski, Janusz