Carbon-fiber microelectrodes for in vivo applications.
Carbon-fiber microelectrodes for in vivo applications.
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DOI：
10.1039/b807563h
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发表时间：
2009-01
期刊：
影响因子：
--
通讯作者：
Venton BJ
中科院分区：
文献类型：
--
作者：
Huffman ML;Venton BJ
碳纤维微电极(CFME)因其体积小、灵敏度高和良好的电化学性质而成为测量神经递质快速变化的有用工具。在这篇文章中，我们重点介绍了利用CFME测量体内神经递质的最新进展。多巴胺一直是人们感兴趣的主要神经递质，但对包括一氧化氮和腺苷在内的其他神经化学物质的直接电化学检测也进行了研究。人们已经研究了表面处理来提高电极的灵敏度，例如共价修饰或添加一层碳纳米管。检测非电活性化合物的酶修饰微电极进一步扩大了CFME的用途，超越了传统的单胺类化合物。CFME继续在体内用于了解基本的神经生物学机制和包括滥用药物在内的药理制剂的作用。在灵敏度和仪器方面的进步现在允许CFME用于测量在行为实验期间发生的自然多巴胺释放。一种在单个微电极上结合电化学和电生理学的新技术有助于更好地了解神经递质浓度及其对细胞放电的影响。未来在这一领域的研究可能集中在制造更小的电极和电极阵列，以及扩大CFME在神经科学中的使用，而不是多巴胺。
Carbon-fiber microelectrodes (CFMEs) have been a useful tool for measuring rapid changes in neurotransmitters because of their small size, sensitivity, and good electrochemical properties. In this article, we highlight recent advances using CFMEs for measuring neurotransmitters in vivo. Dopamine has been a primary neurotransmitter of interest but direct electrochemical detection of other neurochemicals including nitric oxide and adenosine has also been investigated. Surface treatments have been studied to enhance electrode sensitivity, such as covalent modification or the addition of a layer of carbon nanotubes. Enzyme-modified microelectrodes that detect non-electroactive compounds further extend the usefulness of CFMEs beyond the traditional monoamines. CFMEs continue to be used in vivo to understand basic neurobiological mechanisms and the actions of pharmacological agents, including drugs of abuse. Advances in sensitivity and instrumentation now allow CFMEs to be used for measurements of natural dopamine release that occur during behavioral experiments. A new technique combining electrochemistry with electrophysiology at a single microelectrode facilitates a better understanding of neurotransmitter concentrations and their effects on cell firing. Future research in this field will likely concentrate on fabricating smaller electrodes and electrode arrays, as well as expanding the use of CFMEs in neuroscience beyond dopamine.
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影响因子：
13.1
作者：
Burmeister, JJ;Gerhardt, GA
通讯作者：
Gerhardt, GA
影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
简易制备碳纳米管-离子液体凝胶修饰微电极的电化学特性及其在生物电化学中的应用
影响因子：
5.4
作者：
Liu, Ying;Zou, Xiangqin;Dong, Shaojun
通讯作者：
Dong, Shaojun
影响因子：
5.4
作者：
Musameh, M;Wang, J;Lin, YH
通讯作者：
Lin, YH
影响因子：
16.2
作者：
Cheer, Joseph F.;Aragona, Brandon J.;Wightman, R. Mark
通讯作者：
Wightman, R. Mark