A study on the activity of dermal multipotent stem cells in initiation of wound repair
A study on the activity of dermal multipotent stem cells in initiation of wound repair
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真皮多能干细胞在伤口修复启动中的活性研究
DOI：
10.4236/jbise.2013.62014
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发表时间：
2013-02
期刊：
影响因子：
--
通讯作者：
屈纪富
中科院分区：
文献类型：
--
作者：
屈纪富
背景：创伤愈合是一个细胞与细胞、细胞与细胞外基质相互作用的过程。真皮多能干细胞（dMSC）具有促进存活和伤口愈合的能力，但dMSC在伤口愈合中的潜在功能，特别是在伤口修复的启动中，尚未完全了解。方法：从新生大鼠真皮中分离、纯化、扩增dMSCs和成纤维细胞。流式细胞仪检测细胞周期，MTT法检测细胞放射敏感性。在大鼠背部皮肤上切一个7cm的切口，在切口后第1、2、3、4天，将两块直径为1cm、厚度为0.4cm的无菌聚乙烯醇海绵经切口中线插入每只大鼠背部皮下，收集伤口液。采用MTT法检测伤口液对dMSC和成纤维细胞增殖的影响。通过测定体外培养的dMSC的漂浮细胞百分率和向创面迁移的细胞百分率，观察dMSC在创面的粘附和贴壁能力以及对创面液的迁移反应。结果：分离的dMSCs形态均一，增殖能力强。大多数培养的dMSCs是静止的，有少量凋亡细胞。与成纤维细胞相比，dMSCs对辐射更敏感，对伤口液，特别是伤后第1天收集的伤口液的增殖反应更强。此外，它们的附着、粘附和迁移能力在早期伤口流体中显著增强。结论：作为原始干细胞，dMSCs对伤口液体非常敏感，这表明dMSCs在伤口愈合中，特别是在启动伤口修复中的重要作用。
Background: Wound healing is a process of cell-cell interaction and cell-extracellular matrix interaction. Dermal multipotent stem cells (dMSCs) have the abilities to promote survival and wound healing, but the potential function of dMSCs in wound healing, particularly in the initiation of wound repair, has not been fully understood. Methods: dMSCs and fibroblasts were isolated from neonatal rat dermis and were further purified and expanded. The cell cycles were determined with flow cytometry, while the radiosensitivity was measured by MTT assay. Rats were wounded with a 7-cm incision on the back skin and the wound fluids were collected by inserting two pieces of sterile polyvinyl alcohol sponge (1 cmin diameter and0.4 cmin thickness) subcutaneously into the dorsum of each rat through the midline of incision on the 1st, 2nd, 3rd and 4th day after incision. The effects of wound fluids on the proliferation of dMSCs and fibroblasts were measured with MTT assays. dMSC’s abilities of adhesion and attachment and its migration in response to wound fluids collected on the 1st day after incision were explored by measuring the percentage of floating cells and the cells migrated into wounding area in vitro, respectively. Results: The isolated dMSCs were morphologically homogenous and highly proliferative. Most of the cultured dMSCs were quiescent with few apoptotic cells. Compared with fibroblasts, dMSCs were more sensitive to radiation and more proliferative in response to wound fluids, especially to the wound fluids collected on the 1st day after wounding. Moreover, their abilities to attach, adhere and migrate were significantly enhanced with the early-phase wound fluids. Conclusions: As primitive stem cells, dMSCs are very responsive to wound fluids, which suggests dMSCs’ important role in wound healing, especially in initiating wound repair.
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影响因子：
2
作者：
Jifu Qu;T. Cheng;C. Shi;Yuan Lin;X. Ran
通讯作者：
Jifu Qu;T. Cheng;C. Shi;Yuan Lin;X. Ran
影响因子：
3.8
作者：
V. Dvonch;R. Murphey;J. Matsuoka;Gary R. Grotendorst
通讯作者：
V. Dvonch;R. Murphey;J. Matsuoka;Gary R. Grotendorst
影响因子：
4.5
作者：
C. Hillyer
通讯作者：
C. Hillyer
影响因子：
21.3
作者：
Toma, JG;Akhavan, M;Miller, FD
通讯作者：
Miller, FD
影响因子：
10.3
作者：
Amano, S;Akutsu, N;Nishiyama, T
通讯作者：
Nishiyama, T