Identification of a new myotropic decapeptide from the skin secretion of the red-eyed leaf frog, Agalychnis callidryas.
Identification of a new myotropic decapeptide from the skin secretion of the red-eyed leaf frog, Agalychnis callidryas.
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从红眼叶蛙 Agalychnis callidryas 的皮肤分泌物中鉴定出一种新的肌效十肽
DOI：
10.1371/journal.pone.0243326
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Wu D
中科院分区：
文献类型：
--
作者：
Gao Y;Li R;Yang W;Zhou M;Wang L;Ma C;Xi X;Chen T;Shaw C;Wu D
缓激肽相关肽（BRPs）家族是源自蛙皮分泌物的最重要的促肌肽家族之一。在此，从红眼叶蛙（Agalychnis callidryas）中分离并鉴定出一种新型的BRP——卡利肽（callitide），其具有非典型的一级结构FRPAILVRPK - NH₂。成熟肽在N端的一个经典的前肽转化酶切割位点（-KR -）处被切割，并且在C端，一个不寻常的 - GKGKGK序列被去除，利用第一个G残基作为位于C端的K残基的酰胺供体。此后，对卡利肽的合成复制品进行了促肌活性评估，结果显示其能使膀胱显著收缩，半数有效浓度（EC₅₀）值为0.63 nM，比大多数已发现的促肌肽活性更强。通过分子对接和模拟进一步推测了其结合模式。结果表明卡利肽的C端可能选择性地与缓激肽受体B2（BKRB2）结合。未来需要对卡利肽进行进一步研究，以将其开发为潜在的药物先导物。
Bradykinin-related peptides (BRPs) family is one of the most significant myotropic peptide families derived from frog skin secretions. Here, a novel BRP callitide was isolated and identified from the red-eyed leaf frog, Agalychnis callidryas, with atypical primary structure FRPAILVRPK-NH2. The mature peptide was cleaved N-terminally at a classic propeptide convertase cleavage site (-KR-) and at the C-terminus an unusual -GKGKGK sequence was removed using the first G residue as an amide donor for the C-terminally-located K residue. Thereafter, the synthetic replicates of callitide were assessed the myotropic activity and showed a significant contraction of balder, with the 0.63 nM EC50 value, more potent than most discovered myotropic peptides. The binding mode was further speculated by molecular docking and stimulation. The result indicated that the C-terminal of callitide might selectively bind to bradykinin receptor B2 (BKRB2). Further investigation of the callitide needs to be done in the future to be exploited as potential future drug leads.
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DOI：
10.1006/bbrc.2001.5359
发表时间：
2001-08-17
影响因子：
3.1
作者：
Lai, R;Liu, H;Zhang, Y
通讯作者：
Zhang, Y
影响因子：
2
作者：
Thompson, Alan Hunter;Bjourson, Anthony John;McClean, Stephen
通讯作者：
McClean, Stephen
影响因子：
14.9
作者：
Wiederstein, Markus;Sippl, Manfred J
通讯作者：
Sippl, Manfred J
影响因子：
4.4
作者：
Thompson, Alan Hunter;Bjourson, Anthony John;McClean, Stephen
通讯作者：
McClean, Stephen
DOI：
10.3390/molecules21121667
发表时间：
2016-12-03
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Gao Y;Wu D;Xi X;Wu Y;Ma C;Zhou M;Wang L;Yang M;Chen T;Shaw C
通讯作者：
Shaw C