An economical single-sided antibody incubation method for Western blotting.
An economical single-sided antibody incubation method for Western blotting.
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DOI:
10.1016/j.jviromet.2010.07.026
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发表时间:
2010-11
影响因子:
3.1
通讯作者:
Bishop CE
中科院分区:
文献类型:
--
作者:
Pan C;Lan X;Chen H;Bishop CE
A simple, single-sided antibody method for incubating primary and secondary antibodies in Western blotting was developed, which generates significant savings on the use of antibodies. Compared with the conventional immersion technique for antibody incubation, the present economical single-sided antibody incubation method resulted in a saving of 80% of antibody use. Besides, the present incubation method did not compromise the Western blot results and was not affected by the expression levels of target proteins.
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DOI:
10.1073/pnas.76.7.3116
发表时间:
1979-01-01
影响因子:
11.1
作者:
RENART, J;REISER, J;STARK, GR
通讯作者:
STARK, GR
DOI:
10.1007/978-1-59745-542-8_42
发表时间:
2009-01-01
期刊:
PROTEIN BLOTTING AND DETECTION: METHODS AND PROTOCOLS
影响因子:
--
作者:
Dorri, Yaser;Khalili, Ali W.;Scofield, R. Hal
通讯作者:
Scofield, R. Hal
DOI:
10.1073/pnas.76.9.4350
发表时间:
1979-01-01
影响因子:
11.1
作者:
TOWBIN, H;STAEHELIN, T;GORDON, J
通讯作者:
GORDON, J
DOI:
10.1007/978-1-59745-542-8_15
发表时间:
2009-01-01
期刊:
PROTEIN BLOTTING AND DETECTION: METHODS AND PROTOCOLS
影响因子:
--
作者:
Canzler, Ulrich;Bartsch, Holger;Bachmann, Michael
通讯作者:
Bachmann, Michael
影响因子:
3.7
作者:
Canzler, U.;Bartsch, H.;Bachmann, M. P.
通讯作者:
Bachmann, M. P.