An economical single-sided antibody incubation method for Western blotting.

An economical single-sided antibody incubation method for Western blotting.
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DOI:
10.1016/j.jviromet.2010.07.026
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发表时间:
2010-11
影响因子:
3.1
通讯作者:
Bishop CE
Bishop CE
中科院分区:
医学4区
文献类型:
--
作者:
Pan C;Lan X;Chen H;Bishop CE

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开发了一种简单的单面抗体方法,用于在Western blotting中孵育一次和二次抗体,大大节省了抗体的使用。与传统的浸泡抗体孵育方法相比,目前经济的单面抗体孵育方法节省了80%的抗体使用量。此外,该孵育方法不影响Western印迹结果,也不受目的蛋白表达水平的影响。
A simple, single-sided antibody method for incubating primary and secondary antibodies in Western blotting was developed, which generates significant savings on the use of antibodies. Compared with the conventional immersion technique for antibody incubation, the present economical single-sided antibody incubation method resulted in a saving of 80% of antibody use. Besides, the present incubation method did not compromise the Western blot results and was not affected by the expression levels of target proteins.
DOI: 10.1007/978-1-59745-542-8_42
发表时间: 2009-01-01
期刊: PROTEIN BLOTTING AND DETECTION: METHODS AND PROTOCOLS
影响因子: --
作者:
Dorri, Yaser;Khalili, Ali W.;Scofield, R. Hal
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DOI: 10.1073/pnas.76.9.4350
发表时间: 1979-01-01
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影响因子: 3.7
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