Dynamic Observation of Retinal Response to Pressure Elevation in a Microfluidic Chamber.
Dynamic Observation of Retinal Response to Pressure Elevation in a Microfluidic Chamber.
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DOI：
10.1021/acs.analchem.1c05652
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发表时间：
2022-09-13
影响因子：
7.4
通讯作者：
Li, Deyu
中科院分区：
文献类型：
--
作者：
Esteban-Linares, Alberto;Wareham, Lauren K.;Walmsley, Thayer S.;Holden, Joseph M.;Fitzgerald, Matthew L.;Pan, Zhiliang;Xu, Ya-Qiong;Li, Deyu
动态观察细胞和组织对高压的反应有助于我们理解与压力损伤相关的重要生理和病理过程。在这里，我们报告了一种微流体平台，该平台能够保持广泛的稳定工作压力（30至200 mmHg），同时使用低流速（2-14 μl/hr）来限制剪切应力。这是通过迫使流动通过由微流体腔室下游的琼脂糖凝胶组成的多孔阻力基质来实现的。研究了流动特性，并提取了四种不同浓度的琼脂糖的渗透率，与文献中的结果一致。为了证明该装置的能力，我们测量了整个小鼠视网膜中视网膜神经节细胞的细胞内Ca 2+水平响应于压力的变化。平均而言，增加的压力的开始导致视网膜神经节细胞的细胞内Ca 2+水平立即增加119.16%。所展示的微流体平台可广泛用于探测细胞和组织对高压的反应。
Dynamic observation of cell and tissue responses to elevated pressure could help our understanding of important physiological and pathological processes related to pressure-induced injury. Here we report on a microfluidic platform capable of maintaining a wide range of stable operating pressures (30 to 200 mmHg), while using low flowrate (2–14 μl/hr) to limit shear stress. This is achieved by forcing flow through a porous resistance matrix composed of agarose gel downstream of a microfluidic chamber. The flow characteristics were investigated and the permeabilities of the agarose with four different concentrations were extracted, agreeing well with results found in literature. To demonstrate the capability of the device, we measured the change in intracellular Ca2+ levels of retinal ganglion cells in whole mouse retina in response to pressure. Onset of enhanced pressure results in, on average, an immediate 119.16% increase in the intracellular Ca2+ levels of retinal ganglion cells. The demonstrated microfluidic platform could be widely used to probe cell and tissue responses to elevated pressure.
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DOI：
10.3390/molecules15031168
发表时间：
2010-03-03
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Duncan RS;Goad DL;Grillo MA;Kaja S;Payne AJ;Koulen P
通讯作者：
Koulen P
影响因子：
4.1
作者：
Abdulhussain, Noor;Nawada, Suhas;Schoenmakers, Peter
通讯作者：
Schoenmakers, Peter
影响因子：
3.9
作者：
Ahearne, M;Yang, Y;Liu, KK
通讯作者：
Liu, KK
DOI：
10.1115/1.429656
发表时间：
2000-06-01
影响因子：
1.7
作者：
Mauck, RL;Soltz, MA;Ateshian, GA
通讯作者：
Ateshian, GA
影响因子：
3
作者：
Angelucci, A;Clasca, F;Sur, M
通讯作者：
Sur, M