Immunity onset alters plant chromatin and utilizes EDA16 to regulate oxidative homeostasis.
Immunity onset alters plant chromatin and utilizes EDA16 to regulate oxidative homeostasis.
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DOI：
10.1371/journal.ppat.1009572
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发表时间：
2021-05
期刊：
影响因子：
6.7
通讯作者：
Ntoukakis V
中科院分区：
文献类型：
--
作者：
Pardal AJ;Piquerez SJM;Dominguez-Ferreras A;Frungillo L;Mastorakis E;Reilly E;Latrasse D;Concia L;Gimenez-Ibanez S;Spoel SH;Benhamed M;Ntoukakis V
植物对微生物的感知导致转录组的动态重编程，这对植物健康至关重要。这种转录反应的适当幅度可以在多个水平上调节，包括染色质。然而，染色质重塑和转录动力学之间的相互作用，激活植物免疫的机制仍然知之甚少。在这里，我们提出的证据表明，激活植物免疫细菌导致核小体重新定位，这与改变转录。核小体重塑遵循不同基因座处核小体重新定位的不同模式。使用反向遗传筛选，我们鉴定了多种染色质重塑ATP酶，这些酶在免疫中具有以前未描述的作用，包括胚囊发育停滞16、EDA 16。免疫诱导染色质重塑ATP酶EDA 16的功能表征揭示了负调节免疫激活和限制氧化还原稳态变化的机制。我们的转录组学数据与EDA 16功能敲除和过表达突变体的MNase-seq数据相结合，表明EDA 16通过核小体重新定位选择性地调节参与氧化还原信号传导的基因的定义子集。因此，总的来说，染色质重塑ATP酶微调免疫反应，并提供了一个以前未表征的免疫调节机制。免疫信号传导受到严格控制，以避免不适当的激活导致严重的发育惩罚。在微生物的感知之后，启动多个信号级联，导致免疫的转录激活。这种反应的幅度可以在多个水平上调节，包括染色质。在这里，我们表明，激活植物免疫影响核小体定位在数千个位点，并与免疫相关基因的转录。染色质重塑ATP酶的反向遗传筛选确定了六个在植物免疫中具有新作用的基因。我们进一步表征了EDA 16作为免疫应答的负调节剂的作用。EDA 16表达在免疫激活后被诱导，并通过核小体重新定位调节参与氧化还原稳态的基因子集。
Perception of microbes by plants leads to dynamic reprogramming of the transcriptome, which is essential for plant health. The appropriate amplitude of this transcriptional response can be regulated at multiple levels, including chromatin. However, the mechanisms underlying the interplay between chromatin remodeling and transcription dynamics upon activation of plant immunity remain poorly understood. Here, we present evidence that activation of plant immunity by bacteria leads to nucleosome repositioning, which correlates with altered transcription. Nucleosome remodeling follows distinct patterns of nucleosome repositioning at different loci. Using a reverse genetic screen, we identify multiple chromatin remodeling ATPases with previously undescribed roles in immunity, including EMBRYO SAC DEVELOPMENT ARREST 16, EDA16. Functional characterization of the immune-inducible chromatin remodeling ATPase EDA16 revealed a mechanism to negatively regulate immunity activation and limit changes in redox homeostasis. Our transcriptomic data combined with MNase-seq data for EDA16 functional knock-out and over-expressor mutants show that EDA16 selectively regulates a defined subset of genes involved in redox signaling through nucleosome repositioning. Thus, collectively, chromatin remodeling ATPases fine-tune immune responses and provide a previously uncharacterized mechanism of immune regulation. Immune signaling is tightly controlled to avoid inappropriate activation leading to severe developmental penalties. Following the perception of microbes, multiple signaling cascades are initiated leading to transcriptional activation of immunity. The amplitude of this response can be regulated at multiple levels, including chromatin. Here we show that activation of plant immunity affects nucleosome positioning over thousands of loci and is correlated with the transcription of immune-related genes. A reverse genetic screen of chromatin remodeling ATPases identified six genes with novel roles in plant immunity. We further characterize the role of EDA16 as a negative regulator of immune responses. EDA16 expression is induced upon activation of immunity and regulates a subset of genes involved in redox homeostasis through nucleosome repositioning.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
27.5
作者：
Berriri S;Gangappa SN;Kumar SV
通讯作者：
Kumar SV
DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
影响因子：
7.4
作者：
Korves, TM;Bergelson, J
通讯作者：
Bergelson, J
DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dowen, Robert H.;Pelizzola, Mattia;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.