Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness.
Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness.
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DOI：
10.3389/fbioe.2021.596154
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发表时间：
2021
影响因子：
5.7
通讯作者：
Ethier CR
中科院分区：
文献类型：
--
作者：
Boazak EM;King R;Wang J;Chu CM;Toporek AM;Sherwood JM;Overby DR;Geisert EE;Ethier CR
角膜和巩膜的生物力学特性在多种眼部病变的发生和发展中很重要，并且在个体之间存在很大差异，但这种差异的来源仍不清楚。在这里，我们通过对BXD重组近交系小鼠进行高保真眼顺应性测量并进行定量性状分析，确定了推定调节角膜巩膜生物力学组织特性的基因。我们发现了与眼顺应性相关的7个顺式eqtl和非同义snp，并通过RT-qPCR和免疫标记显示，只有两个候选基因Smarce1和Tns4在角膜和巩膜组织中显著表达。两者都具有影响组织材料特性的发展和/或调节的机制潜力。这项工作激发了对Smarce1和tsn4在涉及角膜巩膜的眼部病理中的作用的进一步研究，以及开发新的眼部病理生理小鼠模型，如近视和青光眼。
The biomechanical properties of the cornea and sclera are important in the onset and progression of multiple ocular pathologies and vary substantially between individuals, yet the source of this variation remains unknown. Here we identify genes putatively regulating corneoscleral biomechanical tissue properties by conducting high-fidelity ocular compliance measurements across the BXD recombinant inbred mouse set and performing quantitative trait analysis. We find seven cis-eQTLs and non-synonymous SNPs associating with ocular compliance, and show by RT-qPCR and immunolabeling that only two of the candidate genes, Smarce1 and Tns4, showed significant expression in corneal and scleral tissues. Both have mechanistic potential to influence the development and/or regulation of tissue material properties. This work motivates further study of Smarce1 and Tns4 for their role(s) in ocular pathology involving the corneoscleral envelope as well as the development of novel mouse models of ocular pathophysiology, such as myopia and glaucoma.
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